Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model

Yongkun Zhao; Chong Wang; Boning Qiu; Chufang Li; Hualei Wang; Hongli Jin; Weiwei Gai; Xuexing Zheng; Tiecheng Wang; Weiyang Sun; Feihu Yan; Yuwei Gao; Qian Wang; Jinghua Yan; Ling Chen; Stanley Perlman; Nanshan Zhong; Jincun Zhao; Songtao Yang; Xianzhu Xia

doi:10.1016/j.antiviral.2016.11.016

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Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model

Journal article

Open access

Peer reviewed

Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model

Yongkun Zhao, Chong Wang, Boning Qiu, Chufang Li, Hualei Wang, Hongli Jin, Weiwei Gai, Xuexing Zheng, Tiecheng Wang, Weiyang Sun, …

Antiviral Research, Vol.137, pp.125-130

01/2017

DOI: 10.1016/j.antiviral.2016.11.016

PMID: 27890674

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Abstract

Middle East Respiratory Syndrome (MERS) is a highly lethal pulmonary infection caused by a coronavirus (CoV), MERS-CoV. With the continuing spread of MERS-CoV, prophylactic and therapeutic treatments are urgently needed. In this study, we prepared purified equine F(ab’)2 from horses immunized with MERS-CoV virus-like particles (VLPs) expressing MERS-CoV S, M and E proteins. Both IgG and F(ab’)2 efficiently neutralized MERS-CoV replication in tissue culture. Passive transfer of equine immune antibodies significantly reduced virus titers and accelerated virus clearance from the lungs of MERS-CoV infected mice. Our data show that horses immunized with MERS-CoV VLPs can serve as a primary source of protective F(ab’)2 for potential use in the prophylactic or therapeutic treatment of exposed or infected patients. •Healthy horses immunized with MERS-CoV virus-like particles rapidly generate high titers of virus neutralizing antibodies.•Passive transfer of equine immune antibodies significantly reduced virus titers from the lungs of MERS-CoV infected mice.•F(ab’)2 fragments prepared by digestion of antibody with pepsin to reduce possible allergic responses.•Equine immune antibodies are polyclonal and recognize more antigen determinants in MERS-CoV spike protein.

Animal model

Immunoglobulin

F(ab’)2 fragment

Equine immune serum

Middle East Respiratory Syndrome coronavirus

Details

Title: Subtitle: Passive immunotherapy for Middle East Respiratory Syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model
Creators: Yongkun Zhao - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Chong Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Boning Qiu - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Chufang Li - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Hualei Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Hongli Jin - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Weiwei Gai - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Xuexing Zheng - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Tiecheng Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Weiyang Sun - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Feihu Yan - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Yuwei Gao - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Qian Wang - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Jinghua Yan - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing, China
Ling Chen - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Stanley Perlman - Departments of Microbiology, University of Iowa, Iowa City, USA
Nanshan Zhong - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Jincun Zhao - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
Songtao Yang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Xianzhu Xia - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China
Resource Type: Journal article
Publication Details: Antiviral Research, Vol.137, pp.125-130
DOI: 10.1016/j.antiviral.2016.11.016
PMID: 27890674
NLM abbreviation: Antiviral Res
ISSN: 0166-3542
eISSN: 1872-9096
Publisher: Elsevier B.V
Grant note: name: National Key R&D program, award: 2016YFC1200902; name: National Science and Technology Major Project of the Ministry of Science and Technology of China, award: 2014ZX09102044-007; name: Municipal Healthcare Joint-Innovation Major Project of Guangzhou, award: 201604020011; name: Open Project of the State Key Laboratory of Respiratory Disease and the National Institutes of Health
Language: English
Date published: 01/2017
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777348502771

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