Journal article
Patterning of papillae on the mouse tongue: A system for the quantitative assessment of planar cell polarity signaling
Developmental biology, Vol.419(2), pp.298-310
11/15/2016
DOI: 10.1016/j.ydbio.2016.09.004
PMID: 27612405
Abstract
The dorsal surface of the mouse tongue is covered by ~7000 papillae, asymmetric epithelial protrusions that are precisely oriented to create a stereotyped macroscopic pattern. Within the context of this large-scale pattern, neighboring papillae exhibit a high degree of local order that minimizes the differences in their orientations. We show here that the orientations of lingual papillae are under the control of the core planar cell polarity (PCP) genes Vangl1, Vangl2, and Celsr1. Using K14-Cre and Nkx2.5-Cre to induce conditional knockout of Vangl1 and/or Vangl2 in the tongue epithelium, we observe more severe disruptions to local order among papillae with inactivation of larger numbers of Vangl genes, a greater role for Vangl2 than Vangl1, and a more severe phenotype with the Vangl2 Looptail (Lp) allele than the Vangl2 null allele, consistent with a dominant negative mode of action of the Vangl2Lp allele. Interestingly, Celsr1−/− tongues show disruption of both local and global order, with many papillae in the anterior tongue showing a reversed orientation. To quantify each of these phenotypes, we have developed and applied three procedures for sampling the orientations of papillae and assessing the degree of order on different spatial scales. The experiments reported here establish the dorsal surface of the mouse tongue as a favorable system for studying PCP control of epithelial patterning.
•Lingual papillae exhibit a precise and evolutionarily conserved pattern.•Planar cell polarity genes Vangl1, Vangl2, and Celsr control papillae orientation.•Papillae orientations can be scored for quantitative analyses of polarity defects.•The dorsal surface of the tongue is an attractive system for studying polarity.
Details
- Title: Subtitle
- Patterning of papillae on the mouse tongue: A system for the quantitative assessment of planar cell polarity signaling
- Creators
- Yanshu Wang - Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USAJohn Williams - Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USAAmir Rattner - Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USAShu Wu - Department of Neurology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA, USAAlexander G Bassuk - Department of Neurology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA, USAAndre M Goffinet - Institute of Neuroscience, University of Louvain Medical School and WELBIO, B1200 Brussels, BelgiumJeremy Nathans - Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.419(2), pp.298-310
- DOI
- 10.1016/j.ydbio.2016.09.004
- PMID
- 27612405
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000011, name: Howard Hughes Medical Institute, United States, ; name: National Eye Institute (NIH), United States, , award: 1R01EY018637; name: National Institute of Neurologic Disease and Stroke, award: 1R01NS098590-01; name: Actions de Recherches Concertées, award: ARC-10/15-026; name: Interuniversity Poles of Attraction, award: PDR-FNRS T0002.13; name: Région Wallonne, award: WELBIO-CR-2012A-07
- Language
- English
- Date published
- 11/15/2016
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics); Internal Medicine
- Record Identifier
- 9984013117402771
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