Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina

Jelena Ostojić; Siniša D Grozdanić; Nasreen A Syed; Mark S Hargrove; James T Trent III; Markus H Kuehn; Young H Kwon; Randy H Kardon; Donald S Sakaguchi

doi:10.1001/archopht.126.11.1530

Back

Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina

Journal article

Open access

Peer reviewed

Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina

Jelena Ostojić, Siniša D Grozdanić, Nasreen A Syed, Mark S Hargrove, James T Trent III, Markus H Kuehn, Young H Kwon, Randy H Kardon and Donald S Sakaguchi

Archives of ophthalmology (1960), Vol.126(11), pp.1530-1536

11/2008

DOI: 10.1001/archopht.126.11.1530

PMID: 19001220

Files and links (1)

url

https://doi.org/10.1001/archopht.126.11.1530View

Published (Version of record) Open Access

Abstract

To determine the distribution of 2 intracellular oxygen-carrying molecules, neuroglobin (NGB) and cytoglobin (CYGB), in specific retinal cell types of human retinas. Specific antibodies against NGB and CYGB were used in immunohistochemical studies to examine their distribution patterns in human retinal sections. Double-labeling studies were performed with the anti-NGB and anti-CYGB antibodies along with antibodies against neuronal (microtubule-associated protein 2, class III beta-tubulin [TUJ1], protein kinase C alpha, calretinin) and glial (vimentin, glial fibrillary acid protein) markers. Confocal microscopy was used to examine the retinal sections. Immunohistochemical analysis of human retinal tissue showed NGB and CYGB immunoreactivity in the ganglion cell layer, inner nuclear layer, inner and outer plexiform layers, and retinal pigment epithelium. Neuroglobin immunoreactivity was also present in the outer nuclear layer and photoreceptor inner segments. Neuroglobin and CYGB were coexpressed in the neurons in the ganglion cell layer and inner nuclear layer but not within glial cells. Neuroglobin and CYGB are colocalized within human retinal neurons and retinal pigment epithelium but not within glial cells. Clinical Relevance Our results suggest that NGB and CYGB may serve a neuroprotective role as scavengers of reactive oxygen species and therefore should be considered when developing therapeutic strategies for treatment of hypoxia-related ocular diseases.

Retina - metabolism

Protein Kinase C-alpha - metabolism

Microtubule-Associated Proteins - metabolism

Vimentin - metabolism

Humans

Middle Aged

Infant

Globins - metabolism

Glial Fibrillary Acidic Protein - metabolism

Retinal Ganglion Cells - metabolism

Blotting, Western

Oxygen - metabolism

Nerve Tissue Proteins - metabolism

Pigment Epithelium of Eye - metabolism

S100 Calcium Binding Protein G - metabolism

Microscopy, Confocal

Tubulin - metabolism

Calbindin 2

Aged, 80 and over

Child

Fluorescent Antibody Technique, Indirect

Details

Title: Subtitle: Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina
Creators: Jelena Ostojić - Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
Siniša D Grozdanić
Nasreen A Syed
Mark S Hargrove
James T Trent III
Markus H Kuehn
Young H Kwon
Randy H Kardon
Donald S Sakaguchi
Resource Type: Journal article
Publication Details: Archives of ophthalmology (1960), Vol.126(11), pp.1530-1536
DOI: 10.1001/archopht.126.11.1530
PMID: 19001220
NLM abbreviation: Arch Ophthalmol
ISSN: 0003-9950
eISSN: 1538-3601
Publisher: American Medical Association; United States
Grant note: NS44007 / NINDS NIH HHS
Language: English
Date published: 11/2008
Academic Unit: Pathology; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980060402771

Metrics

33 Record Views

26 Times Cited - Web of Science