Journal article
Pemphigus Foliaceus and Pemphigus Vulgaris Autoantibodies React with the Extracellular Domain of Desmoglein-1
Journal of investigative dermatology, Vol.104(3), pp.323-328
03/1995
DOI: 10.1111/1523-1747.ep12665364
PMID: 7860995
Abstract
Pemphigus foliaceus is associated with an autoimmune response against desmoglein-1; however, the fine specificity of these autoantibodies and the role that they play in pathogenesis have not yet been elucidated. In an attempt to develop a system to facilitate the detection and characterization of this antigen/antibody system, recombinant human desmoglein-1 was expressed in COS-1 cells, a mammalian epithelial cell line. The desmoglein-1 transgene product was shown to be expressed on the surface of the COS-1 cells in the appropriate transmembrane orientation. All pemphigus foliaceus sera (endemic form, n = 24; nonendemic form, n = 7) reacted strongly with nonpermeabilized desmoglein-1-transfected cells, exhibiting a punctate cell-surface staining pattern. This reactivity against the desmoglein-1 ectodomain was predominantly an IgG4-restricted response and was calcium dependent. Ten of 18 pemphigus vulgaris sera also reacted with the extracellular domain of recombinant desmoglein-1. Use of this eukaryotic expression system should greatly facilitate further characterization of the anti-desmoglain-1 autoimmune response associated with pemphigus faliaceus and pemphigus vulgaris and may aid in determining its pathogenic relevance.
Details
- Title: Subtitle
- Pemphigus Foliaceus and Pemphigus Vulgaris Autoantibodies React with the Extracellular Domain of Desmoglein-1
- Creators
- Daryl J Emery - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.ALuis K Diaz - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.AJanet A Fairley - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.AArgelia Lopez - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.AAnn F Taylor - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.AGeorg J Giudice - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.104(3), pp.323-328
- Publisher
- Elsevier Inc
- DOI
- 10.1111/1523-1747.ep12665364
- PMID
- 7860995
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 03/1995
- Academic Unit
- Dermatology
- Record Identifier
- 9984025592602771
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