Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: permeability characteristics, toxin distribution, and tissue damage

Catherine C Davis; Mary J Kremer; Patrick M Schlievert; Christopher A Squier

doi:10.1016/S0002-9378(03)00873-1

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Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: permeability characteristics, toxin distribution, and tissue damage

Journal article

Peer reviewed

Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: permeability characteristics, toxin distribution, and tissue damage

Catherine C Davis, Mary J Kremer, Patrick M Schlievert and Christopher A Squier

American journal of obstetrics and gynecology, Vol.189(6), pp.1785-1791

12/2003

DOI: 10.1016/S0002-9378(03)00873-1

PMID: 14710116

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Abstract

The purpose of this study was to evaluate transvaginal penetration of toxic shock syndrome toxin-1 and its effects on permeability and tissue integrity in vitro with the use of excised porcine vaginal mucosa. Permeability to tritiated water (1 and 10 microg/mL applied toxin) and transmucosal flux of (35)S-methionine-labeled toxic shock syndrome toxin-1 (10 and 20 microg/mL) for up to 12 hours were assessed with the use of a continuous flow perfusion system. The location of labeled toxin that penetrated the mucosal tissue strata was determined. The integrity of toxin-treated, intact, scalpel-incised tissue was evaluated histopathologically. Toxic shock syndrome toxin-1 caused a non-dose-dependent increase in mucosal permeability and traversed the intact mucosa at a low rate without disrupting tissue integrity. In incised vaginal mucosa, toxic shock syndrome toxin-1 induced subepithelial separation and atrophy that were analogous to clinically relevant vaginal lesions that were reported in fatal cases of menstrual toxic shock syndrome. An in vitro model could be used to demonstrate that toxic shock syndrome toxin-1 permeates the vaginal mucosa and distributes throughout the tissue. Histologic evaluation of tissues that were exposed to toxic shock syndrome toxin-1 demonstrated lesions that were similar to those lesions that were reported in cases of menstrual toxic shock syndrome.

Immunohistochemistry

Shock, Septic - pathology

Enterotoxins - toxicity

Culture Techniques

Vagina - drug effects

Mucous Membrane - pathology

Animals

Bacterial Toxins

Swine

Cell Membrane Permeability - drug effects

Sensitivity and Specificity

Superantigens

Female

Mucous Membrane - drug effects

Enterotoxins - pharmacology

Vagina - pathology

Details

Title: Subtitle: Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: permeability characteristics, toxin distribution, and tissue damage
Creators: Catherine C Davis - Procter & Gamble Company, Cincinnati, Ohio, USA
Mary J Kremer
Patrick M Schlievert
Christopher A Squier
Resource Type: Journal article
Publication Details: American journal of obstetrics and gynecology, Vol.189(6), pp.1785-1791
DOI: 10.1016/S0002-9378(03)00873-1
PMID: 14710116
NLM abbreviation: Am J Obstet Gynecol
ISSN: 0002-9378
eISSN: 1097-6868
Publisher: United States
Language: English
Date published: 12/2003
Academic Unit: Oral Pathology, Radiology and Medicine; Microbiology and Immunology; International Programs; Internal Medicine
Record Identifier: 9984001128102771

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