Journal article
Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells
PloS one, Vol.6(11), pp.e27609-e27609
2011
DOI: 10.1371/journal.pone.0027609
PMCID: PMC3217999
PMID: 22110688
Abstract
Penicillium marneffei (P. marneffei) is considered an indicator pathogen of AIDS, and the endemicity and clinical features of P. marneffei have been described. While, how the co-infection of P. marneffei exacerbate deterioration of the immune response remains poorly understood. Here we isolated P. marneffei from the cutaneous lesions of AIDS patients and analyzed its effects on HIV-1-dendritic cells (DCs) interaction. We demonstrated that the monocyte-derived dendritic cells (MDDCs) could be activated by both thermally dimorphic forms of P. marneffei for significantly promoting HIV-1 trans-infection of CD4(+) T cells, while these activated MDDCs were refractory to HIV-1 infection. Mechanistically, P. marneffei-activated MDDCs endocytosed large amounts of HIV-1 and sequestrated the internalized viruses into tetrapasnin CD81(+) compartments potentially for proteolysis escaping. The activated MDDCs increased expression of intercellular adhesion molecule 1 and facilitated the formation of DC-T-cell conjunctions, where much more viruses were recruited. Moreover, we found that P. marneffei-stimulated MDDCs efficiently activated resting CD4(+) T cells and induced more susceptible targets for viral infection. Our findings demonstrate that DC function and its interaction with HIV-1 have been modulated by opportunistic pathogens such as P. marneffei for viral dissemination and infection amplification, highlighting the importance of understanding DC-HIV-1 interaction for viral immunopathogenesis elucidation.
Details
- Title: Subtitle
- Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells
- Creators
- Yan Qin - Key Laboratory of Molecular Virology & ImmunologyYuye Li - The First Affiliated Hospital of Kunming Medical CollegeWan Liu - Key Laboratory of Molecular Virology & ImmunologyRenrong Tian - Key Laboratory of Molecular Virology & ImmunologyQianqian Guo - Key Laboratory of Molecular Virology & ImmunologyShaoyou Li - The First Affiliated Hospital of Kunming Medical CollegeHongbin Li - The First Affiliated Hospital of Kunming Medical CollegeDaojun Zhang - The First Affiliated Hospital of Kunming Medical CollegeYongtang Zheng - Key Laboratory of Animal Models and Human Disease MechanismsLi Wu - Department of Veterinary BioscienceKe Lan - Key Laboratory of Molecular Virology & ImmunologyJianhua Wang - Key Laboratory of Molecular Virology & Immunology
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.6(11), pp.e27609-e27609
- DOI
- 10.1371/journal.pone.0027609
- PMID
- 22110688
- PMCID
- PMC3217999
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science
- Language
- English
- Date published
- 2011
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001227502771
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