Journal article
Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells
Vaccine, Vol.29(15), pp.2705-2711
2011
DOI: 10.1016/j.vaccine.2011.01.079
PMCID: PMC3084484
PMID: 21310190
Abstract
Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.
Details
- Title: Subtitle
- Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells
- Creators
- Frances M Saccoccio - Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United StatesAnne L Sauer - Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United StatesXiaohong Cui - Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United StatesAmy E Armstrong - Department of Medicine, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United StatesEL-Sayed E Habib - Department of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura, EgyptDavid C Johnson - Department of Molecular Microbiology and Immunology, Oregon Health & Sciences University, Portland, OR 97239, United StatesBrent J Ryckman - Division of Biological Sciences, University of Montana, Missoula, MT 59812, United StatesAloysius J Klingelhutz - Department of Microbiology, University of Iowa, 2202 MERF, 375 Newton Road, Iowa City, IA 52242, United StatesStuart P Adler - Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United StatesMichael A McVoy - Department of Pediatrics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, VA 23298, United States
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.29(15), pp.2705-2711
- DOI
- 10.1016/j.vaccine.2011.01.079
- PMID
- 21310190
- PMCID
- PMC3084484
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2011
- Academic Unit
- Microbiology and Immunology; Radiation Oncology
- Record Identifier
- 9984001148602771
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