Perforin-deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-gamma but requires TNF-alpha

Douglas W White; John T Harty

doi:10.4049/jimmunol.160.2.898

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Perforin-deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-gamma but requires TNF-alpha

Journal article

Peer reviewed

Perforin-deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-gamma but requires TNF-alpha

Douglas W White and John T Harty

The Journal of immunology (1950), Vol.160(2), pp.898-905

01/15/1998

DOI: 10.4049/jimmunol.160.2.898

PMID: 9551927

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Abstract

CD8+ T cells are effective mediators of immunity against Listeria monocytogenes, but the mechanisms by which they provide antilisterial immunity are poorly understood. CD8+ T cells efficiently lyse target cells in vitro by at least two independent pathways. To test the hypothesis that CD8+ T cell-mediated immunity to L. monocytogenes is dependent on perforin or CD95 (Fas, Apo-1), we used C57BI/6 (B6) and perforin-deficient (PO) mice to generate CD8+ T cell lines specific for the L. mono cytogenes-encoded Ag listeriolysin O (LLO). Both lines specifically produce IFN-gamma and TNF-alpha, and mediate target cell lysis in vitro. Cytolysis mediated by the PO-derived CD8+ T cell line is delayed relative to the B6-derived line and is completely inhibited by anti-CD95 Abs. In vivo, PO-derived CD8+ T cells provide specific antilisterial immunity in B6 hosts, CD95-deficient hosts, and IFN-gamma-depleted hosts. However, PO-derived CD8+ T cells fail to provide antilisterial immunity in hosts depleted of TNF-alpha. These results indicate that single Ag-specific CD8+ T cells derived from PO mice can mediate antilisterial immunity by a mechanism that is independent of CD95 or IFN-gamma, but requires TNF-alpha.

Cytotoxicity Tests, Immunologic

Spleen - immunology

CD8-Positive T-Lymphocytes - transplantation

Tumor Necrosis Factor-alpha - genetics

Adoptive Transfer

Listeria monocytogenes - immunology

Tumor Necrosis Factor-alpha - deficiency

Mice, Inbred MRL lpr

Bacterial Toxins

Listeriosis - immunology

CD8-Positive T-Lymphocytes - metabolism

Pore Forming Cytotoxic Proteins

fas Receptor - physiology

Epitopes, T-Lymphocyte - immunology

Interferon-gamma - genetics

Interferon-gamma - deficiency

Perforin

Hemolysin Proteins

Mice, Inbred C57BL

Interferon-gamma - physiology

Spleen - cytology

Heat-Shock Proteins - immunology

Membrane Glycoproteins - genetics

Mice, Knockout

Animals

Tumor Necrosis Factor-alpha - physiology

Mice

CD8-Positive T-Lymphocytes - immunology

Membrane Glycoproteins - deficiency

Cytotoxicity, Immunologic

Details

Title: Subtitle: Perforin-deficient CD8+ T cells provide immunity to Listeria monocytogenes by a mechanism that is independent of CD95 and IFN-gamma but requires TNF-alpha
Creators: Douglas W White - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City 52242, USA
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.160(2), pp.898-905
DOI: 10.4049/jimmunol.160.2.898
PMID: 9551927
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: AI07511 / NIAID NIH HHS AI36864 / NIAID NIH HHS
Language: English
Date published: 01/15/1998
Academic Unit: Pathology
Record Identifier: 9984047605902771

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