Journal article
Perfusion of the placenta assessed using arterial spin labeling and ferumoxytol dynamic contrast enhanced magnetic resonance imaging in the rhesus macaque
Magnetic resonance in medicine, Vol.81(3), pp.1964-1978
03/2019
DOI: 10.1002/mrm.27548
PMCID: PMC6715150
PMID: 30357902
Abstract
To investigate the correspondence between arterial spin labeling (ASL) flow-sensitive alternating inversion recovery (FAIR) and ferumoxytol DCE MRI for the assessment of placental intervillous perfusion.
Ten pregnant macaques in late second trimester were imaged at 3 T using a 2D ASL FAIR, with and without outer-volume saturation pulses used to control the bolus width, and a 3D ferumoxytol DCE-MRI acquisition. The ASL tagged/control pairs were averaged, subtracted, and normalized to create perfusion ratio maps. Contrast arrival time and uptake slope were estimated by fitting the DCE data to a sigmoid function. Macaques (N = 4) received interleukin-1β to induce inflammation and disrupt perfusion.
The FAIR tag modification with outer-volume saturation reduced the median ASL ratio percentage compared with conventional FAIR (0.64% ± 1.42% versus 0.71% ± 2.00%; P < .05). Extended ferumoxytol arrival times (34 ± 25 seconds) were observed across the placenta. No significant DCE signal change was measured in fetal tissue (
0.6% ± 3.0%; P = .52) or amniotic fluid (1.9% ± 8.8%; P = .59). High ASL ratio was significantly correlated with early arrival time and high uptake slope (P < .05), but ASL signal was not above noise in late-DCE-enhancing regions. No significant differences were observed in perfusion measurements between the interleukin-1β and controls (P > .05).
The ASL-FAIR and ferumoxytol DCE-MRI methods are feasible to detect early blood delivery to the macaque placenta. Outer volume saturation reduced the high macrovascular ASL signal. Interleukin-1β exposure did not alter placental intervillous perfusion. An endogenous-labeling perfusion technique is limited due to extended transit times for flow within the placenta beyond the immediate vicinity of the maternal spiral arteries.
Details
- Title: Subtitle
- Perfusion of the placenta assessed using arterial spin labeling and ferumoxytol dynamic contrast enhanced magnetic resonance imaging in the rhesus macaque
- Creators
- Kai D Ludwig - University of Wisconsin–MadisonSean B Fain - University of Wisconsin–MadisonSydney M Nguyen - University of Wisconsin–MadisonThaddeus G Golos - University of Wisconsin–MadisonScott B Reeder - University of Wisconsin–MadisonIan M Bird - University of Wisconsin–MadisonDinesh M Shah - University of Wisconsin–MadisonOliver E Wieben - University of Wisconsin–MadisonKevin M Johnson - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Magnetic resonance in medicine, Vol.81(3), pp.1964-1978
- DOI
- 10.1002/mrm.27548
- PMID
- 30357902
- PMCID
- PMC6715150
- NLM abbreviation
- Magn Reson Med
- ISSN
- 0740-3194
- eISSN
- 1522-2594
- Grant note
- NICHD U01HD087216 / National Institutes for Health (NIH) Human Placental Project (HPP) P51 OD011106 / NIH HHS U01 HD087216 / NICHD NIH HHS Pharmaceuticals Wisconsin Alumni Research Foundation T32 HD041921 / NICHD NIH HHS T32 CA009206 / NCI NIH HHS TL1 TR000429 / NCATS NIH HHS GE Healthcare UL1 TR000427 / NCATS NIH HHS Office of the Vice Chancellor for Research K24 DK102595 / NIDDK NIH HHS TL1 TR002375 / NCATS NIH HHS School of Medicine
- Language
- English
- Date published
- 03/2019
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health, Sport, and Human Physiology
- Record Identifier
- 9984275058702771
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