Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion

Ki Yong Hong; Hosung Bae; Intae Park; Dae-Young Park; Kyun Hoo Kim; Yoshiaki Kubota; Eui-Sic Cho; Hail Kim; Ralf H Adams; Ook-Joon Yoo; Gou Young Koh

doi:10.1242/dev.125336

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Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion

Journal article

Peer reviewed

Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion

Ki Yong Hong, Hosung Bae, Intae Park, Dae-Young Park, Kyun Hoo Kim, Yoshiaki Kubota, Eui-Sic Cho, Hail Kim, Ralf H Adams, Ook-Joon Yoo, …

Development (Cambridge), Vol.142(15), pp.2623-2632

08/01/2015

DOI: 10.1242/dev.125336

PMID: 26243869

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Abstract

Despite the growing interest in adipose tissue as a therapeutic target of metabolic diseases, the identity of adipocyte precursor cells (preadipocytes) and the formation of adipose tissue during embryonic development are still poorly understood. Here, we clarified the identity and dynamic processes of preadipocytes in mouse white adipose tissue during embryogenesis through direct examination, lineage tracing and culture systems. Surprisingly, we found that lipid-lacking but perilipin(+) or adiponectin(+) proliferating preadipocytes started to emerge at embryonic day 16.5, and these cells underwent active proliferation until birth. Moreover, these preadipocytes resided as clusters and were distributed along growing adipose vasculatures. Importantly, the embryonic preadipocytes exhibited considerable coexpression of stem cell markers, such as CD24, CD29 and PDGFRα, and a small portion of preadipocytes were derived from PDGFRβ(+) mural cells, in contrast to the adult preadipocytes present in the stromal vascular fraction. Further analyses with in vitro and ex vivo culture systems revealed a stepwise but dynamic regulation of preadipocyte formation and differentiation during prenatal adipogenesis. To conclude, we unraveled the identity and characteristics of embryonic preadipocytes, which are crucial for the formation and expansion of adipose tissue during embryogenesis.

3T3-L1 Cells

Adipocytes - metabolism

Adipose Tissue - blood supply

Adipose Tissue - embryology

Animals

Azo Compounds

Carrier Proteins - metabolism

CD24 Antigen - metabolism

Cell Proliferation - physiology

Colony-Forming Units Assay

Flow Cytometry

Galactosides

Indoles

Integrin beta1 - metabolism

Mice

Mice, Inbred C57BL

Mice, Transgenic

Microscopy, Confocal

Perilipin-1

Phosphoproteins - metabolism

Real-Time Polymerase Chain Reaction

Receptor, Platelet-Derived Growth Factor alpha - metabolism

Statistics, Nonparametric

Details

Title: Subtitle: Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion
Creators: Ki Yong Hong - Korea Advanced Institute of Science and Technology
Hosung Bae - Korea Advanced Institute of Science and Technology
Intae Park - Korea Advanced Institute of Science and Technology
Dae-Young Park - Korea Advanced Institute of Science and Technology
Kyun Hoo Kim - Korea Advanced Institute of Science and Technology
Yoshiaki Kubota - Keio University
Eui-Sic Cho - Jeonbuk National University
Hail Kim - Korea Advanced Institute of Science and Technology
Ralf H Adams - University of Münster
Ook-Joon Yoo - Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea gykoh@kaist.ac.kr ojyoo@kaist.ac.kr
Gou Young Koh - Korea Advanced Institute of Science and Technology
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.142(15), pp.2623-2632
DOI: 10.1242/dev.125336
PMID: 26243869
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Language: English
Date published: 08/01/2015
Academic Unit: Internal Medicine
Record Identifier: 9985217028702771

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