Journal article
Peritoneal Cell-Free Tumor DNA as Biomarker for Peritoneal Surface Malignancies
Annals of surgical oncology, Vol.27(13), pp.5065-5071
12/2020
DOI: 10.1245/s10434-020-08832-9
PMCID: PMC9731140
PMID: 32648179
Abstract
Disease burden in patients with peritoneal carcinomatosis (PC) is difficult to estimate. We evaluate whether peritoneal cell-free tumor DNA can be used as a measure of disease burden.
Malignant ascites or peritoneal lavage fluids were collected from patients with PC under approved IRB protocol. Cell-free DNA was extracted from peritoneal fluid. Droplet digital PCR (ddPCR) was performed using a commercially available KRAS G12/G13 screening kit. Mutant allele frequency (MAF) was calculated based on the numbers of KRAS wild-type and mutant droplets. Clinicopathological, treatment and outcome data were abstracted and correlated with MAF of cell-free KRAS mutant DNA.
Cell-free KRAS mutant DNA was detected in 15/37 (40%) malignant peritoneal fluids with a MAF of 0.1% to 26.2%. While peritoneal cell-free KRAS mutant DNA was detected in all the patients with KRAS mutant tumors (N = 10), 3/16 (19%) patients with KRAS wild-type tumors also had peritoneal cell-free KRAS mutant DNA. We also found that 71% (5/7) of patients with disease amenable to cytoreductive surgery (CRS) had a MAF of < 1% (median: 0.5%, range: 0.1-4.7%), while 75% (6/8) of patients with unresectable disease had a MAF of > 1% (median: 4.4%, range: 0.1-26.2%).
This pilot proof-of-principle study suggests that peritoneal cell-free tumor DNA detected by ddPCR may enable prediction of disease burden and a measure of disease amenable to CRS in patients with PC.
Details
- Title: Subtitle
- Peritoneal Cell-Free Tumor DNA as Biomarker for Peritoneal Surface Malignancies
- Creators
- Katie M Leick - University of IowaAustin G Kazarian - University of IowaMaheen Rajput - University of IowaAnn Tomanek-Chalkley - University of IowaAnn Miller - University of IowaHannah R Shrader - University of IowaAshley McCarthy - University of IowaKristen L Coleman - University of IowaPashtoon M Kasi - University of IowaCarlos H F Chan - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Annals of surgical oncology, Vol.27(13), pp.5065-5071
- DOI
- 10.1245/s10434-020-08832-9
- PMID
- 32648179
- PMCID
- PMC9731140
- NLM abbreviation
- Ann Surg Oncol
- ISSN
- 1068-9265
- eISSN
- 1534-4681
- Grant note
- T35 HL007485 / NHLBI NIH HHS T32 CA078586 / NIH HHS
- Language
- English
- Date published
- 12/2020
- Academic Unit
- Radiology; Microbiology and Immunology; Hematology, Oncology, and Blood & Marrow Transplantation; Surgery; Radiation Oncology; Holden Comprehensive Cancer Center; Internal Medicine
- Record Identifier
- 9984313086702771
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