Journal article
Permeabilization reveals classical stimulus-secretion coupling in bovine parathyroid cells
Endocrinology (Philadelphia), Vol.121(4), pp.1571-1576
1987
DOI: 10.1210/endo-121-4-1571
PMID: 2820703
Abstract
Unlike most secretory cells, high extracellular Ca2+ inhibits, rather than stimulates, PTH release from parathyroid cells. To gain more direct access to the potentially unique secretory apparatus of this cell type, we developed techniques for permeabilizing parathyroid cells using electroshock. Exposure of dispersed bovine parathyroid cells to five 2-kV discharges results in more than 90% uptake of trypan blue and rapid loss of intracellular 86Rb, documenting permeabilization of the plasma membrane. Unlike intact parathyroid cells, a high Ca2+ concentration increases PTH release 2.8-fold from permeabilized cells, with half-maximal and maximal stimulation at 10-5 and 4 × 10-4 M Ca2+, respectively, a pattern similar to classical stimulus-secretion coupling. Moreover, PTH release was low at Ca2+ concentrations equivalent to those in intact cells at low extracellular Ca2+ levels (-2 × 10-7 M). Cellular mechanisms other than changes in the cytosolic Ca2+ concentration per se, therefore, presumably mediate low Ca2+-stimulated PTH release in the intact cell. Diacylglycerol and protein kinase C may play a role in this process, since dioctanoylglycerol (10-4 M) and 12- O-tetradecanoyl phorbol 13-acetate (10-6 M) each enhance PTH release from permeabilized cells 2- to 4-fold at a Ca2+ concentration (-2 × 10-7 M) equivalent to that present in the intact cell at low extracellular Ca2+ concentrations. © 1987 by The Endocrine Society.
Details
- Title: Subtitle
- Permeabilization reveals classical stimulus-secretion coupling in bovine parathyroid cells
- Creators
- M Oetting - Duke UniversityM. S Leboff - Brigham and Women's HospitalS Levy - Brigham and Women's HospitalL Swiston - Brigham and Women's HospitalJ Preston - Brigham and Women's HospitalC Chen - Brigham and Women's HospitalE. M Brown - Brigham and Women's Hospital
- Resource Type
- Journal article
- Publication Details
- Endocrinology (Philadelphia), Vol.121(4), pp.1571-1576
- Publisher
- Endocrine Society
- DOI
- 10.1210/endo-121-4-1571
- PMID
- 2820703
- ISSN
- 0013-7227
- eISSN
- 1945-7170
- Language
- English
- Date published
- 1987
- Academic Unit
- Stead Family Department of Pediatrics; General Pediatrics and Adolescent Medicine
- Record Identifier
- 9984353933902771
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