Journal article
Peroxidase as a model for reduction of tertiary amine oxides catalyzed by rat hepatic supernatant and microsomal fractions
Biochemical pharmacology, Vol.38(4), pp.573-579
1989
DOI: 10.1016/0006-2952(89)90201-3
PMID: 2917014
Abstract
Rat hepatic microsomal and 100,000
g supernatant fractions catalyzed an NADH- and FMN-dependent reduction of amine oxides. Horseradish peroxidase (HRP) served as a model for the amine oxide reductase located in rat hepatic 100,000
g supernatant fraction. The HRP-catalyzed reaction displayed saturation kinetics with respect to NADH and the amine oxide substrate; however, there was an optimum concentration for FMN after which inhibition was observed at increased concentrations of FMN. The reductase in the 100,000
g hepatic supernatant fraction closely paralleled HRP-catalyzed amine oxide reduction in coenzyme requirements, sensitivity to inhibitors, and substrate specificity. Moreover, the peroxidase activity of HRP and microsomal and 100,000
g supernatant fractions correlated with the NADH- and FMN-dependent amine oxide reductase activities of these enzyme preparations. The NADH- and FMN-dependent amine oxide reductase activity in 100,000
g supernatant fractions, however, did not parallel the aldehyde oxidase activity. Thus, the results indicate that there is an amine oxide reductase in rat hepatic 100,000
g supernatant fraction with catalytic properties that are modeled well by horseradish peroxidase.
Details
- Title: Subtitle
- Peroxidase as a model for reduction of tertiary amine oxides catalyzed by rat hepatic supernatant and microsomal fractions
- Creators
- Stephen G. Gillespie - University of IowaMichael W. Duffel - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Biochemical pharmacology, Vol.38(4), pp.573-579
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0006-2952(89)90201-3
- PMID
- 2917014
- ISSN
- 0006-2952
- eISSN
- 1873-2968
- Language
- English
- Date published
- 1989
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303161802771
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