Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy

Dieanira Erudaitius; Andrew Huang; Sarah Kazmi; Garry R Buettner; Victor G J Rodgers

doi:10.1371/journal.pone.0170442

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Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy

Journal article

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Peer reviewed

Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy

Dieanira Erudaitius, Andrew Huang, Sarah Kazmi, Garry R Buettner and Victor G J Rodgers

PloS one, Vol.12(1), pp.e0170442-e0170442

2017

DOI: 10.1371/journal.pone.0170442

PMCID: PMC5249139

PMID: 28107421

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Abstract

Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.

Immunohistochemistry

Aquaporin 3 - metabolism

Cell Survival

Humans

Ascorbic Acid - pharmacology

Gene Silencing

Hydrogen Peroxide - pharmacology

Cell Line, Tumor

Neoplasms - pathology

Aquaporin 3 - genetics

Details

Title: Subtitle: Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy
Creators: Dieanira Erudaitius - Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America
Andrew Huang - Department of Neuroscience, University of California, Riverside, Riverside, California, United States of America
Sarah Kazmi - Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America
Garry R Buettner - Free Radical & Radiation Biology, Department of Radiation Oncology, University of Iowa College of Medicine, Iowa City, IA, United States of America
Victor G J Rodgers - Department of Bioengineering, University of California, Riverside, Riverside, California, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.12(1), pp.e0170442-e0170442
DOI: 10.1371/journal.pone.0170442
PMID: 28107421
PMCID: PMC5249139
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: P30 ES005605 / NIEHS NIH HHS P30 CA086862 / NCI NIH HHS R01 CA169046 / NCI NIH HHS
Language: English
Date published: 2017
Academic Unit: Radiation Oncology
Record Identifier: 9984047875502771

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