Peroxisome proliferators alter lipid acquisition and elastin gene expression in neonatal rat lung fibroblasts

Stephen E McGowan; Sheila K Jackson; Melissa M Doro; Paula J Olson

doi:10.1152/ajplung.1997.273.6.L1249

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Peroxisome proliferators alter lipid acquisition and elastin gene expression in neonatal rat lung fibroblasts

Journal article

Open access

Peer reviewed

Peroxisome proliferators alter lipid acquisition and elastin gene expression in neonatal rat lung fibroblasts

Stephen E McGowan, Sheila K Jackson, Melissa M Doro and Paula J Olson

The American journal of physiology, Vol.273(6), pp.L1249-L1257

12/1997

DOI: 10.1152/ajplung.1997.273.6.L1249

PMID: 9435581

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Abstract

During the alveolar stage of lung development, lipid droplet-laden interstitial cells are present at the base of elongating alveolar septa. These cells that have been named lipid interstitial cells or lipofibroblasts (LFs) may supply lipids for surfactant production, the synthesis of membrane phospholipids, and/or energy metabolism. They also have myofibroblastic characteristics and participate in the generation of the interstitial elastic fiber network, that is, in the pulmonary alveolar septum. To understand how this cell regulates its lipid-storing and elastin-producing properties, we have examined the effects of peroxisome proliferators on the expression of the genes that are associated with an elastin-producing myofibroblastic phenotype or an adipocyte-like phenotype. Two known ligands for peroxisome proliferator-activated receptors, 5,8,11,14-eicosatetraynoic acid (ETYA) and 15-deoxy-delta-12,14-prostaglandin J2 (15-dPGJ2), decrease elastin gene transcription and the steady-state levels of tropoelastin (TE) and alpha-smooth muscle actin mRNAs in cultured LFs. Concurrently, cultured LFs increase the expression of adipocyte lipid binding protein, which is regarded as an adipocyte-specific protein, and accumulate lipid droplets. Their abilities to store lipids and express desmin intermediate filaments, alpha-smooth muscle actin, and smooth muscle myosin heavy chain in contractile filaments in vitro illustrate similarities among the pulmonary LF, the hepatic lipocyte, and the contractile interstitial cell, which contribute to the repair reaction in the lung after pulmonary injury.

Lipid Metabolism

Animals, Newborn

Recombinant Fusion Proteins - biosynthesis

Transcription, Genetic - drug effects

Transcription Factors - physiology

Actins - biosynthesis

Cells, Cultured

Microbodies - drug effects

Rats

Receptors, Cytoplasmic and Nuclear - physiology

5,8,11,14-Eicosatetraynoic Acid - pharmacology

Lung - cytology

Gene Expression Regulation - drug effects

RNA, Messenger - biosynthesis

Animals

Fibroblasts - drug effects

Elastin - biosynthesis

Prostaglandin D2 - analogs & derivatives

Fibroblasts - cytology

Lung - metabolism

Prostaglandin D2 - pharmacology

Cytoskeletal Proteins - biosynthesis

Fibroblasts - metabolism

Details

Title: Subtitle: Peroxisome proliferators alter lipid acquisition and elastin gene expression in neonatal rat lung fibroblasts
Creators: Stephen E McGowan - Department of Veterans Affairs Research Service, Iowa City, Iowa, USA
Sheila K Jackson
Melissa M Doro
Paula J Olson
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.273(6), pp.L1249-L1257
DOI: 10.1152/ajplung.1997.273.6.L1249
PMID: 9435581
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Publisher: American Physiological Society; United States
Language: English
Date published: 12/1997
Academic Unit: International Programs; Internal Medicine
Record Identifier: 9983984522502771

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