Journal article
Personalized proteomics for the diagnosis and treatment of idiopathic inflammatory disease
JAMA ophthalmology, Vol.134(4), pp.444-448
04/01/2016
DOI: 10.1001/jamaophthalmol.2015.5934
PMCID: PMC4833518
PMID: 26848019
Abstract
Importance To better characterize posterior uveitis, vitreous samples from 15 patients were subjected to antibody arrays, and the expression levels of 200 human cytokines were evaluated. Expression was analyzed by 1-way analysis of variance (significance at P < .01), unsupervised cluster algorithm, and pathway analysis.
Observations Unbiased clustering of patients, based on their cytokine expression profile, suggested that particular protein networks and molecular pathways are altered in various forms of uveitis. Expression of interleukin 23 (IL-23), IL-1 receptor I (IL-1RI), IL-17R, tissue inhibitors of metalloproteinase 1 and 2 (TIMP-1 and TIMP-2), insulinlike growth factor–binding protein 2 (IGFBP-2), nerve growth factor (b-NGF), platelet-derived growth factor receptor β polypeptide (PDGFRb), bone morphogenic protein 4 (BMP-4), and stem cell factor (SCF) constituted a common cytokine signature in the vitreous of patients with uveitis. In 1 patient with progressive, idiopathic visual loss, this last-line analysis implicated retinal autoimmunity, a diagnosis that was validated when her serum sample was found to contain antibodies to S-arrestin, a retinal protein and potent cause of autoimmune retinal degeneration.
Conclusions and Relevance The analysis identifies a common cytokine signature for posterior uveitis and guides the diagnosis of a patient with idiopathic uveitis. Personalized treatment reversed the visual loss, illustrating how proteomic tools may individualize therapy
Details
- Title: Subtitle
- Personalized proteomics for the diagnosis and treatment of idiopathic inflammatory disease
- Creators
- Gabriel VelezC. Nathaniel RoybalDiana ColganStephen H TsangAlexander G BassukVinit B Mahajan
- Resource Type
- Journal article
- Publication Details
- JAMA ophthalmology, Vol.134(4), pp.444-448
- DOI
- 10.1001/jamaophthalmol.2015.5934
- PMID
- 26848019
- PMCID
- PMC4833518
- NLM abbreviation
- JAMA Ophthalmol
- ISSN
- 2168-6165
- eISSN
- 2168-6173
- Publisher
- American Medical Association
- Language
- English
- Date published
- 04/01/2016
- Description audience
- Professional
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics); Internal Medicine
- Record Identifier
- 9984020772902771
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