Journal article
Personalized viral genomic investigation of herpes simplex virus 1 perinatal viremic transmission with dual fatality
Cold Spring Harbor molecular case studies, Vol.5(6), p.a004382
12/2019
DOI: 10.1101/mcs.a004382
PMCID: PMC6913147
PMID: 31582464
Abstract
Here we present a personalized viral genomics approach to investigating a rare case of perinatal herpes simplex virus 1 (HSV-1) transmission that ended in death of both mother and neonate. We sought to determine whether the virus involved in this rare case had any unusual features that may have contributed to the dire patient outcome. A pregnant woman with negative HerpeSelect antibody test underwent cesarean section at 30 wk gestation and died the same day. The premature newborn died 5 d later. Both individuals were found postmortem to have positive blood HSV-1 PCR tests. Using oligonucleotide enrichment and deep sequencing, we determined that viral transmission from mother to infant was nearly perfect at the consensus genome level. At the virus population level, 77% of minor variants (MVs) in the mother's blood also appeared on the neonate's skin, of which more than half were disseminated into the neonate's blood. We also detected nonmaternal MVs that arose de novo in the neonate's viral populations. Of note, one de novo MV in the neonate's skin virus induced a nonsynonymous mutation in the UL6 protein, which is a component of the portal that allows DNA entry into new progeny capsids. This case suggests that perinatal viremic HSV-1 transmission includes the majority of genetic diversity from the maternal virus population and that new, nonsynonymous mutations can occur after relatively few rounds of replication. This report expands our understanding of viral transmission in humans and may lead to improved diagnostic strategies for neonatal HSV-1 acquisition.
Details
- Title: Subtitle
- Personalized viral genomic investigation of herpes simplex virus 1 perinatal viremic transmission with dual fatality
- Creators
- Mackenzie M Shipley - Pennsylvania State UniversityDaniel W Renner - Pennsylvania State UniversityUtsav Pandey - Pennsylvania State UniversityBradley Ford - University of IowaDavid C Bloom - University of FloridaCharles Grose - University of IowaMoriah L Szpara - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Cold Spring Harbor molecular case studies, Vol.5(6), p.a004382
- DOI
- 10.1101/mcs.a004382
- PMID
- 31582464
- PMCID
- PMC6913147
- NLM abbreviation
- Cold Spring Harb Mol Case Stud
- ISSN
- 2373-2865
- eISSN
- 2373-2873
- Grant note
- R01 AI048633 / NIAID NIH HHS R21 AI140443 / NIAID NIH HHS
- Language
- English
- Date published
- 12/2019
- Academic Unit
- Stead Family Department of Pediatrics; Pathology; Infectious Disease (Pediatrics)
- Record Identifier
- 9984186395202771
Metrics
15 Record Views