Journal article
Pertussis toxin prevents homologous desensitization of adenylate cyclase in cultured renal epithelial cells
The Journal of biological chemistry, Vol.261(4), pp.1503-1506
1986
DOI: 10.1016/S0021-9258(17)35966-5
PMID: 3080428
Abstract
The biochemical mechanisms of adenylate cyclase desensitization in arginine vasopressin-responsive epithelial cells remain unclear. Preincubation of cultured rabbit renal cortical collecting tubular cells with arginine vasopressin leads to a 30-100% decline in arginine vasopressin-stimulated adenylate cyclase activity. This loss of adenylate cyclase activity is time- and arginine vasopressin concentration-dependent. Preincubation with arginine vasopressin does not result in significant changes in basal, NaF-, forskolin-, isoproterenol- or cholera toxin-stimulated adenylate cyclase activity. Preincubation of cells with chlorophenylthio-cAMP, forskolin, and cholera toxin does not result in loss of arginine vasopressin-stimulated adenylate cyclase activity. Since products of cyclo-oxygenase inhibit arginine vasopressin action, cells were preincubated with indomethacin. Arginine vasopressin-induced adenylate cyclase desensitization is not reversed by indomethacin. By contrast, incubation with pertussis toxin prevents arginine vasopressin-induced adenylate cycle desensitization. These data demonstrate that arginine vasopressin induces homologous desensitization in membranes from cultured rabbit cortical collecting tubular cells and suggest that this desensitization is mediated, at least in part, by pertussis toxin substrate. These observations provide a unifying mechanism for desensitization of adenylate cyclase-coupled hormone receptors.
Details
- Title: Subtitle
- Pertussis toxin prevents homologous desensitization of adenylate cyclase in cultured renal epithelial cells
- Creators
- Patricia D Wilson - Univ. Colorado, health sci. center, Denver CO 80262, United StatesBradley S Dixon - Univ. Colorado, health sci. center, Denver CO 80262, United StatesMark A Dillingham - Univ. Colorado, health sci. center, Denver CO 80262, United StatesJ. Adolfo Garcia-Sainz - Univ. Colorado, health sci. center, Denver CO 80262, United StatesRobert J Anderson - Univ. Colorado, health sci. center, Denver CO 80262, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.261(4), pp.1503-1506
- DOI
- 10.1016/S0021-9258(17)35966-5
- PMID
- 3080428
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- American Society for Biochemistry and Molecular Biology
- Language
- English
- Date published
- 1986
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984094770702771
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