Phage typing of Staphylococcus intermedius

G D Overturf; D A Talan; K Singer; N Anderson; J I Miller; R T Greene; S Froman

doi:10.1128/jcm.29.2.373-375.1991

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Phage typing of Staphylococcus intermedius

Journal article

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Phage typing of Staphylococcus intermedius

G D Overturf, D A Talan, K Singer, N Anderson, J I Miller, R T Greene and S Froman

Journal of clinical microbiology, Vol.29(2), pp.373-375

02/1991

DOI: 10.1128/jcm.29.2.373-375.1991

PMCID: PMC269770

PMID: 2007645

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Abstract

Staphylococcus intermedius, a coagulase-positive staphylococcal species, is a common canine pathogen and a rare human wound pathogen. A total of 145 strains of S. intermedius (ATCC 29663, 4 reference strains, 4 human isolates, 44 canine infection isolates, and 92 isolates from canine gingiva) were screened for lysogenic phage by a modified Fisk method. Nineteen phage preparations were prepared for preliminary typing experiments. Lytic activity was observed on 93 of 145 (64.1%) isolates, yielding 44 lytic patterns with individual strains susceptible to one or more phages. Five phages lysed only a single strain, but lytic patterns varied from 1 to 11 lytic phages per isolate. A distinct lytic pattern did not separate canine or human wound isolates from canine gingival isolates. All human wound isolates fell into the two most common canine gingival or wound patterns; the single human nasopharyngeal isolate was not lysed by any phage. Twenty-two of 44 (55%) canine wound isolates and 65 of 92 (71%) gingival isolates yielded lytic patterns. Lysogenic phages are common in S. intermedius. This preliminary study suggests that phage typing may be a useful tool in distinguishing epidemiologically related strains.

Animals

Bacterial Typing Techniques

Bacteriophages - genetics

Dogs

Gingiva - microbiology

Humans

Lysogeny

Staphylococcal Infections - microbiology

Staphylococcus - classification

Staphylococcus - genetics

Staphylococcus - isolation & purification

Wound Infection - microbiology

Details

Title: Subtitle: Phage typing of Staphylococcus intermedius
Creators: G D Overturf - University of California, Los Angeles
D A Talan - University of California, Los Angeles
K Singer - University of California, Los Angeles
N Anderson - University of California, Los Angeles
J I Miller - University of California, Los Angeles
R T Greene - University of California, Los Angeles
S Froman - University of California, Los Angeles
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.29(2), pp.373-375
DOI: 10.1128/jcm.29.2.373-375.1991
PMID: 2007645
PMCID: PMC269770
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 02/1991
Academic Unit: Emergency Medicine; Internal Medicine
Record Identifier: 9984296976602771

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