Journal article
Phenotypic and functional characteristics of HLA-DR + neutrophils in Brazilians with cutaneous leishmaniasis
Journal of leukocyte biology, Vol.101(3), pp.739-749
03/2017
DOI: 10.1189/jlb.4A0915-442RR
PMCID: PMC5295851
PMID: 28076241
Abstract
The protozoan
causes cutaneous leishmaniasis (CL) in endemic regions. In murine models, neutrophils (PMNs) are recruited to the site of infection soon after parasite inoculation. However, the roles of neutrophils during chronic infection and in human disease remain undefined. We hypothesized that neutrophils help maintain a systemic inflammatory state in subjects with CL. Lesion biopsies from all patients with CL tested contained neutrophils expressing HLA-DR, a molecule thought to be restricted to professional antigen-presenting cells. Although CL is a localized disease, a subset of patients with CL also had circulating neutrophils expressing HLA-DR and the costimulatory molecules CD80, CD86, and CD40. PMNs isolated from a low-density leukocyte blood fraction (LD-PMNs) contained a higher percentage of HLA-DR
PMNs than did normal-density PMNs. In vitro coculture experiments suggested LD-PMNs do not suppress T cell responses, differentiating them from MDSCs. Flow-sorted HLA-DR
PMNs morphologically resembled conventional PMNs, and they exhibited functional properties of PMNs. Compared with conventional PMNs, HLA-DR
PMNs showed increased activation, degranulation, DHR123 oxidation, and phagocytic capacity. A few HLA-DR
PMNs were observed in healthy subjects, and that proportion could be increased by incubation in either inflammatory cytokines or in plasma from a patient with CL. This was accompanied by an increase in PMN
mRNA, suggesting a possible connection between neutrophil "priming" and up-regulation of HLA-DR. These data suggest that PMNs that are primed for activation and that also express surface markers of antigen-presenting cells emerge in the circulation and infected tissue lesions of patients with CL.
Details
- Title: Subtitle
- Phenotypic and functional characteristics of HLA-DR + neutrophils in Brazilians with cutaneous leishmaniasis
- Creators
- Richard E Davis - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa, USASmriti Sharma - Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaJacilara Conceição - Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais, INCT-DT (CNPq/MCT), Salvador, Bahia, BrazilPedro Carneiro - Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais, INCT-DT (CNPq/MCT), Salvador, Bahia, BrazilFernanda Novais - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USAPhillip Scott - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USAShyam Sundar - Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaOlivia Bacellar - Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais, INCT-DT (CNPq/MCT), Salvador, Bahia, BrazilEdgar M Carvalho - Fundação Gonçalo Muniz, Fiocruz-Bahia, Salvador, Bahia BrazilMary E Wilson - Research Service, Iowa City Veterans' Affairs Medical Center, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Journal of leukocyte biology, Vol.101(3), pp.739-749
- Publisher
- United States
- DOI
- 10.1189/jlb.4A0915-442RR
- PMID
- 28076241
- PMCID
- PMC5295851
- ISSN
- 0741-5400
- eISSN
- 1938-3673
- Grant note
- R01 AI045540 / NIAID NIH HHS T32 AI007511 / NIAID NIH HHS R01 AI076233 / NIAID NIH HHS I01 BX001983 / BLRD VA I01 BX000536 / BLRD VA P50 AI030639 / NIAID NIH HHS
- Language
- English
- Date published
- 03/2017
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001202602771
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