Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow

Annette J Schlueter; Judith K Glasgow

doi:10.1002/cyto.a.20262

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Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow

Journal article

Open access

Peer reviewed

Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow

Annette J Schlueter and Judith K Glasgow

Cytometry. Part A, Vol.69(4), pp.281-290

04/2006

DOI: 10.1002/cyto.a.20262

PMID: 16528720

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https://doi.org/10.1002/cyto.a.20262View

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Abstract

Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte-related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyte-related subsets in each location. Murine PB and BM cells were simultaneously stained for monocyte-related populations, using five-color flow cytometry. Relevant subsets were defined on the basis of Ly-6C, CD11b, and wheat germ agglutinin phenotype in addition to light-scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors. Six monocyte-related populations were defined, three each in BM and PB. No identical populations were found between the two tissues. Two BM populations and one PB population have heterogeneous expression of many markers, suggesting additional complexity among monocyte-related subsets. The murine monocytic series comprises multiple subsets, differing between PB and BM. This study defines and extensively phenotypes six of these populations, providing preliminary information about possible developmental relationships and migratory capacities of these cells.

Flow Cytometry - methods

Specific Pathogen-Free Organisms

Monocytes - cytology

Mice, Inbred C57BL

Biomarkers - analysis

Antigens, Ly - analysis

Monocytes - chemistry

Phenotype

Animals

CD11b Antigen - analysis

Wheat Germ Agglutinins - analysis

Mice

Mice, Congenic

Details

Title: Subtitle: Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow
Creators: Annette J Schlueter - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, 52242, USA. schluetera@uihc.uiowa.edu
Judith K Glasgow
Resource Type: Journal article
Publication Details: Cytometry. Part A, Vol.69(4), pp.281-290
DOI: 10.1002/cyto.a.20262
PMID: 16528720
NLM abbreviation: Cytometry A
ISSN: 1552-4922
eISSN: 1552-4930
Publisher: United States
Language: English
Date published: 04/2006
Academic Unit: Pathology
Record Identifier: 9984047726302771

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