Journal article
Phorbol ester and dibutyryl cyclic AMP reduce content and efflux of taurine in primary cerebellar astrocytes in culture
Neuroscience letters, Vol.95(1), pp.323-328
1988
DOI: 10.1016/0304-3940(88)90679-9
PMID: 2852322
Abstract
In 16–18 days in vitro (DIV) primary astrocyte cultures prepared from 7- to 9-day-old rats, 48 h exposure to 12,13-phorbol dibutyrate (PDBU) (1 μM) or dibutyryl cAMP (dbcAMP) (1 mM) reduced cellular taurine content, and both basal and 50 mM K
+-evoked taurine efflux, but did not alter cellular glutamate or total protein content. Decreases in cellular taurine content first became apparent between 1 and 6 h and were maximal after 24 h. Treatment also rapidly altered astrocyte morphology to a more processbearing form within 1 h. In contrast, fibroblast growth factor (FGF), epidermal growth factor (EGF), dbcGMP and α-PDBU did not affect cellular morphology, amino acid content or taurine efflux at any time tested. These findings suggest that, while protein kinase C translocation and adenylate cyclase activation may be only indirectly involved in the regulation of astrocyte morphology, long-term decreases in cellular taurine content and efflux may be the more direct result of these second messenger systems.
Details
- Title: Subtitle
- Phorbol ester and dibutyryl cyclic AMP reduce content and efflux of taurine in primary cerebellar astrocytes in culture
- Creators
- Robert A PhilibertGary R Dutton
- Resource Type
- Journal article
- Publication Details
- Neuroscience letters, Vol.95(1), pp.323-328
- DOI
- 10.1016/0304-3940(88)90679-9
- PMID
- 2852322
- NLM abbreviation
- Neurosci Lett
- ISSN
- 0304-3940
- eISSN
- 1872-7972
- Publisher
- Elsevier Ireland Ltd
- Language
- English
- Date published
- 1988
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984003987902771
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