Journal article
Phosphatidylserine inhibits NFκB and p38 MAPK activation in human monocyte derived dendritic cells
Molecular immunology, Vol.48(15-16), pp.1771-1777
09/2011
DOI: 10.1016/j.molimm.2011.04.021
PMID: 21628073
Abstract
► Phosphatidylserine inhibits myeloid derived dendritic cell maturation and function. ► Phosphatidylserine inhibits p38 MAPK activation in dendritic cells treated with LPS. ► Phosphatidylserine inhibits nuclear translocation of NFκB in dendritic cells treated with LPS.
Phosphatidylserine (PS) is an anionic phospholipid restricted to the inner surface of the plasma membrane. PS translocates to the cell surface during early apoptosis where it serves as a marker for rapid uptake by phagocytes. PS is also thought to regulate immune responses. Dendritic cells (DC) are the most potent antigen presenting cells. Previous studies demonstrated that PS inhibits the expression of MHC and co-stimulatory molecules, the secretion of IL-12p70, and the ability to activate T cells by human monocyte derived DCs. However, the cell signaling mechanisms by which PS regulated DCs are not well described. In the current study we tested the effects of PS on signal transduction pathways thought to regulate human myeloid DC maturation and IL-12p70 production. We showed that PS inhibited the activation of nuclear factor-κB (NFκB) in response to LPS by preventing IκBα phosphorylation and degradation. PS also increased the total IκBα levels in immature DCs and inhibited p38 mitogen activated protein kinase (MAPK) phosphorylation and activation. The findings suggest a possible mechanism for regulating the immunostimulatory function of DCs by PS.
Details
- Title: Subtitle
- Phosphatidylserine inhibits NFκB and p38 MAPK activation in human monocyte derived dendritic cells
- Creators
- Kara Doffek - Medical College of WisconsinXiao Chen - Medical College of WisconsinSonia L. Sugg - University of IowaJoel Shilyansky - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular immunology, Vol.48(15-16), pp.1771-1777
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.molimm.2011.04.021
- PMID
- 21628073
- ISSN
- 0161-5890
- eISSN
- 1872-9142
- Grant note
- Wisconsin Breast Cancer Showhouse Children's Hospital Foundation Medical College of Wisconsin Cancer Center Riding for Cure Foundation
- Language
- English
- Date published
- 09/2011
- Academic Unit
- Stead Family Department of Pediatrics; Surgery
- Record Identifier
- 9984322958702771
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