Phospholipase A2 and arachidonate increase in bronchoalveolar lavage fluid after inhaled antigen challenge in asthmatics

D L Bowton; M C Seeds; M B Fasano; B Goldsmith; D A Bass

doi:10.1164/ajrccm.155.2.9032172

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Phospholipase A2 and arachidonate increase in bronchoalveolar lavage fluid after inhaled antigen challenge in asthmatics

Journal article

Peer reviewed

Phospholipase A2 and arachidonate increase in bronchoalveolar lavage fluid after inhaled antigen challenge in asthmatics

D L Bowton, M C Seeds, M B Fasano, B Goldsmith and D A Bass

American journal of respiratory and critical care medicine, Vol.155(2), pp.421-425

02/1997

DOI: 10.1164/ajrccm.155.2.9032172

PMID: 9032172

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Abstract

Phospholipases A2 (PLA2) hydrolyze phospholipids resulting in the release of fatty acids including arachidonic acid (AA) and lysophospholipids. AA, in turn, serves as a substrate for the synthesis of leukotrienes which can cause bronchoconstriction and airways edema and appear to be important mediators of clinical asthma. Further, lysophospholipids may be cytotoxic and/or impair the function of surfactant. We examined the release of secretory PLA2 (sPLA2) and AA into the airways after antigen challenge in 16 subjects with allergic asthma. Asthmatic subjects underwent bronchoscopy with bronchoalveolar lavage (BAL) before and after inhaled antigen challenge; in addition, a single BAL, without inhaled antigen, was performed in 10 control subjects. BAL was obtained at 4 h (n = 7), the time of the late asthmatic response (LAR) (n = 5), or 24 h (n = 4) after challenge. There was no difference between normal and asthmatic subjects in either BAL fluid (BALF) sPLA2 activity or AA concentration at baseline. Both sPLA2 and AA increased after antigen challenge (p < 0.01 and 0.05, respectively). These changes were most marked 4 h after challenge (p < 0.03 for both). sPLA2 may play an important role in the generation of AA in patients with asthma.

Asthma - diagnosis

Arachidonic Acid - metabolism

Asthma - metabolism

Gas Chromatography-Mass Spectrometry

Humans

Bronchial Provocation Tests

Bronchoalveolar Lavage Fluid - chemistry

Phospholipases A - metabolism

Phospholipases A2

Respiratory Function Tests

Methacholine Chloride

Details

Title: Subtitle: Phospholipase A2 and arachidonate increase in bronchoalveolar lavage fluid after inhaled antigen challenge in asthmatics
Creators: D L Bowton - The Department of Internal Medicine, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27157-1054, USA
M C Seeds
M B Fasano
B Goldsmith
D A Bass
Resource Type: Journal article
Publication Details: American journal of respiratory and critical care medicine, Vol.155(2), pp.421-425
DOI: 10.1164/ajrccm.155.2.9032172
PMID: 9032172
ISSN: 1073-449X
eISSN: 1535-4970
Grant note: NHLBI PO1 HL50395 / NHLBI NIH HHS M01 RR07122 / NCRR NIH HHS
Language: English
Date published: 02/1997
Academic Unit: Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology; Immunology; Internal Medicine
Record Identifier: 9984093337202771

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