Journal article
Phospholipids modifications in human hepatoma cell lines (HepG2) exposed to silver and iron oxide nanoparticles
Archives of toxicology, Vol.94(8), pp.2625-2636
08/2020
DOI: 10.1007/s00204-020-02789-0
PMID: 32474618
Abstract
Metallic nanoparticles such as silver (Ag NPs) and iron oxide (Fe3O4 NPs) nanoparticles are high production volume materials due to their applications in various consumer products, and in nanomedicine. However, their inherent toxicities to human cells remain a challenge. The present study was aimed at combining lipidomics data with common phenotypically-based toxicological assays to gain better understanding into cellular response to Ag NPs and Fe3O4 NPs exposure. HepG2 cells were exposed to different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 µg/ml) of the nanoparticles for 24 h, after which they were assayed for toxic effects using toxicological assays like cytotoxicity, mutagenicity, apoptosis and oxidative stress. The cell membrane phospholipid profile of the cells was also performed using shotgun tandem mass spectrometry. The results showed that nanoparticles exposure resulted in concentration-dependent cytotoxicity as well as reduced cytokinesis-block proliferation index (CBPI). Also, there was an increase in the production of ROS and superoxide anions in exposed cells compared to the negative control. The lipidomics data revealed that nanoparticles exposure caused a modulation of the phospholipidome of the cells. A total of 155 lipid species were identified, out of which the fold changes of 23 were significant. The high number of differentially changed phosphatidylcholine species could be an indication that inflammation is one of the major mechanisms of toxicity of the nanoparticles to the cells.
Details
- Title: Subtitle
- Phospholipids modifications in human hepatoma cell lines (HepG2) exposed to silver and iron oxide nanoparticles
- Creators
- Joseph A AdeyemiCarlos Arterio SorgiAna Rita Thomazela MachadoAbayomi T OgunjimiLuiz Gustavo Araujo GardinassiViviani NardiniLucia Helena FaccioliLusania Maria Greggi AntunesFernando Barbosa
- Resource Type
- Journal article
- Publication Details
- Archives of toxicology, Vol.94(8), pp.2625-2636
- DOI
- 10.1007/s00204-020-02789-0
- PMID
- 32474618
- ISSN
- 0340-5761
- eISSN
- 1432-0738
- Grant note
- DOI: 10.13039/501100001807, name: Fundação de Amparo à Pesquisa do Estado de São Paulo, award: 2015/20725-5, 2018/24069-3; DOI: 10.13039/501100002322, name: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, award: 001
- Language
- English
- Date published
- 08/2020
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984066339702771
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