Journal article
Phosphoproteins in extracellular vesicles as candidate markers for breast cancer
Proceedings of the National Academy of Sciences - PNAS, Vol.114(12), pp.3175-3180
03/21/2017
DOI: 10.1073/pnas.1618088114
PMCID: PMC5373352
PMID: 28270605
Abstract
The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. Here we demonstrate a strategy to isolate and identify phosphoproteins in extracellular vesicles (EVs) from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides in EVs isolated from small volumes of plasma samples. Using label-free quantitative phosphoproteomics, we identified 144 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer compared with healthy controls. Several biomarkers were validated in individual patients using paralleled reaction monitoring for targeted quantitation. This study demonstrates that the development of phosphoproteins in plasma EV as disease biomarkers is highly feasible and may transform cancer screening and monitoring.
Details
- Title: Subtitle
- Phosphoproteins in extracellular vesicles as candidate markers for breast cancer
- Creators
- I-Hsuan Chen - Purdue University West LafayetteLiang Xue - Purdue University West LafayetteChuan-Chih Hsu - Purdue University West LafayetteJuan Sebastian Paez Paez - Purdue University West LafayetteLi Pan - Purdue University West LafayetteHillary Andaluz - Purdue University West LafayetteMichael K. Wendt - Purdue University West LafayetteAnton B. Iliuk - Tymora Analytical OperationsJian-Kang Zhu - Purdue University West LafayetteW. Andy Tao - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.114(12), pp.3175-3180
- DOI
- 10.1073/pnas.1618088114
- PMID
- 28270605
- PMCID
- PMC5373352
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- Natl Acad Sciences
- Number of pages
- 6
- Grant note
- R41GM109626 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 1506752 / National Science Foundation; National Science Foundation (NSF) P30CA023168 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P30 CA023168 / Purdue University Center for Cancer Research (NIH Grant) 5R41GM109626; 1R01GM111788; 1R41CA210772 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 03/21/2017
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984460323902771
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