Journal article
Phosphorylation of native and truncated isoforms of protein τ by the double‐stranded DNA‐dependent protein kinase (DNA‐PK) shows that the primary phosphorylation sites are localized between amino acid residues 212‐231 of the longest τ
IUBMB life, Vol.40(1), pp.21-31
09/1996
DOI: 10.1080/15216549600201492
Abstract
Alzheimer's disease (AD) is pathologically characterized by the appearance of neurofibrillary tangles (NFT), senile plaques, and loss of subpopulation of neuronal cells. The NFT is composed of paired helical filaments (PHT) with extensively modified protein τ as its primary constituent.
Previously we had reported on the hyperphosphorylation of τ by a double‐stranded‐DNA‐stimulated protein kinase (DNA‐PK). In this communication, we have compared the DNA‐PK mediated phosphorylation of native and truncated τs with that catalyzed by the cAMP‐dependent protein kinase (PKA). In addition, we have attempted to map the primary site(s) of phosphorylation of τ by DNA‐PK. Our results suggest that DNA‐PK phosphorylates τ at sites substantially different from those targeted by PKA. Furthermore, we show that the primary phosphorylation sites lie between amino acid residues 212‐231 (using numbering system for the longest τ, which is the isoform with four “repeats” and a 58 amino acid “insert” at the carboxyl‐ and amino‐termini, respectively, of the protein molecule).
Details
- Title: Subtitle
- Phosphorylation of native and truncated isoforms of protein τ by the double‐stranded DNA‐dependent protein kinase (DNA‐PK) shows that the primary phosphorylation sites are localized between amino acid residues 212‐231 of the longest τ
- Creators
- Joseph M WuYuping ChenTze‐chen HsiehRoland BrandtGloria Lee
- Resource Type
- Journal article
- Publication Details
- IUBMB life, Vol.40(1), pp.21-31
- Publisher
- Informa Healthcare; UK
- DOI
- 10.1080/15216549600201492
- ISSN
- 1521-6543
- eISSN
- 1521-6551
- Number of pages
- 11
- Language
- English
- Date published
- 09/1996
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984070310402771
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