Journal article
Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
Journal of microscopy (Oxford), Vol.239(2), pp.117-134
08/01/2010
DOI: 10.1111/j.1365-2818.2009.03363.x
PMID: 20629917
Abstract
Lipophilic fluorescent dyes have been used to trace neuronal connections because of their ability to diffuse laterally within nerve cell membranes. Given the hundreds to thousands of connections that a typical neuron makes with its neighbours, a diffusion-matched set of spectrally distinct dyes is desirable. To extend a set of these dyes to obtain six independent labels, we have characterized the properties of novel violet and near-infrared candidates. By combining two-photon and confocal microscopy all of these candidates can be imaged using a single Titanium Sapphire laser. Here we present measurements of the two-photon action cross-sections and diffusion properties of the dyes, using either the relative diffusion distance or fluorescence recovery after photobleaching techniques, and demonstrate six-colour neuronal tracing within the spinal cord and brain tissue.
Details
- Title: Subtitle
- Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
- Creators
- J Tonniges - Creighton University, Department of Physics, Omaha, NE 68178, USAM HansenJ DuncanM J BassettB FritzschB D GrayA EaswaranM G Nichols
- Resource Type
- Journal article
- Publication Details
- Journal of microscopy (Oxford), Vol.239(2), pp.117-134
- DOI
- 10.1111/j.1365-2818.2009.03363.x
- PMID
- 20629917
- NLM abbreviation
- J Microsc
- ISSN
- 0022-2720
- eISSN
- 1365-2818
- Publisher
- England
- Grant note
- 5R01DC005590-07 / NIDCD NIH HHS
- Language
- English
- Date published
- 08/01/2010
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070294902771
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