Journal article
Photopolymerizable Zwitterionic Polymer Patterns Control Cell Adhesion and Guide Neural Growth
Biomacromolecules, Vol.18(8), pp.2389-2401
08/14/2017
DOI: 10.1021/acs.biomac.7b00579
PMCID: PMC6372952
PMID: 28671816
Abstract
Developing materials that reduce or eliminate fibrosis encapsulation of neural prosthetic implants could significantly enhance implant fidelity by improving the tissue/electrode array interface. Here, we report on the photografting and patterning of two zwitterionic materials, sulfobetaine methacrylate (SBMA) and carboxybetaine methacrylate (CBMA), for controlling the adhesion and directionality of cells relevant to neural prosthetics. CBMA and SBMA polymers were photopolymerized and grafted on glass surfaces then characterized by X-ray photoelectron spectroscopy, water contact angle, and protein adsorption. Micropatterned surfaces were fabricated with alternating zwitterionic and uncoated bands. Fibroblasts, cells prevalent in fibrotic tissue, almost exclusively migrate and grow on uncoated bands with little to no cells present on zwitterionic bands, especially for CBMA-coated surfaces. Astrocytes and Schwann cells showed similarly low levels of cell adhesion and morphology changes when cultured on zwitterionic surfaces. Additionally, Schwann cells and inner ear spiral ganglion neuron neurites aligned well to zwitterionic patterns.
Details
- Title: Subtitle
- Photopolymerizable Zwitterionic Polymer Patterns Control Cell Adhesion and Guide Neural Growth
- Creators
- Braden L Leigh - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United StatesElise Cheng - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United StatesLinjing Xu - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United StatesCorinne Andresen - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United StatesMarlan R Hansen - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United StatesC Allan Guymon - Departments of Chemical and Biochemical Engineering, ‡Otolaryngology Head and Neck Surgery, and §Neurosurgery, University of Iowa , Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Biomacromolecules, Vol.18(8), pp.2389-2401
- DOI
- 10.1021/acs.biomac.7b00579
- PMID
- 28671816
- PMCID
- PMC6372952
- NLM abbreviation
- Biomacromolecules
- ISSN
- 1525-7797
- eISSN
- 1526-4602
- Publisher
- United States
- Grant note
- T32 DC000040 / NIDCD NIH HHS UL1 TR002537 / NCATS NIH HHS T35 HL007485 / NHLBI NIH HHS R01 DC012578 / NIDCD NIH HHS P30 DC010362 / NIDCD NIH HHS
- Language
- English
- Date published
- 08/14/2017
- Academic Unit
- Molecular Physiology and Biophysics; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
- Record Identifier
- 9984007284402771
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