Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth

Bradley W Tuft; Shufeng Li; Linjing Xu; Joseph C Clarke; Scott P White; Bradley A Guymon; Krystian X Perez; Marlan R Hansen; C. Allan Guymon

doi:10.1016/j.biomaterials.2012.09.053

Back

Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth

Journal article

Peer reviewed

Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth

Bradley W Tuft, Shufeng Li, Linjing Xu, Joseph C Clarke, Scott P White, Bradley A Guymon, Krystian X Perez, Marlan R Hansen and C. Allan Guymon

Biomaterials, Vol.34(1), pp.42-54

01/2013

DOI: 10.1016/j.biomaterials.2012.09.053

PMCID: PMC4306579

PMID: 23069708

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/4306579View

Open Access

Abstract

Cochlear implants (CIs) provide auditory perception to individuals with severe hearing impairment. However, their ability to encode complex auditory stimuli is limited due, in part, to poor spatial resolution caused by electrical current spread in the inner ear. Directing nerve cell processes towards target electrodes may reduce the problematic current spread and improve stimulatory specificity. In this work, photopolymerization was used to fabricate micro- and nano-patterned methacrylate polymers to probe the extent of spiral ganglion neuron (SGN) neurite and Schwann cell (SGSC) contact guidance based on variations in substrate topographical cues. Micropatterned substrates are formed in a rapid, single-step reaction by selectively blocking light with photomasks which have parallel line-space gratings with periodicities of 10–100 μm. Channel amplitudes of 250 nm–10 μm are generated by modulating UV exposure time, light intensity, and photoinitiator concentration. Gradual transitions are observed between ridges and grooves using scanning electron and atomic force microscopy. The transitions stand in contrast to vertical features generated via etching lithographic techniques. Alignment of neural elements increases significantly with increasing feature amplitude and constant periodicity, as well as with decreasing periodicity and constant amplitude. SGN neurite alignment strongly correlates (r = 0.93) with maximum feature slope. Multiple neuronal and glial types orient to the patterns with varying degrees of alignment. This work presents a method to fabricate gradually-sloping micropatterns for cellular contact guidance studies and demonstrates spatial control of inner ear neural elements in response to micro- and nano-scale surface topography.

Nerve guide

Micropatterning

Surface topography

Photopolymerization

Neural prosthesis

Details

Title: Subtitle: Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth
Creators: Bradley W Tuft - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA
Shufeng Li - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Linjing Xu - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Joseph C Clarke - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Scott P White - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA
Bradley A Guymon - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Krystian X Perez - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA
Marlan R Hansen - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
C. Allan Guymon - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Biomaterials, Vol.34(1), pp.42-54
DOI: 10.1016/j.biomaterials.2012.09.053
PMID: 23069708
PMCID: PMC4306579
NLM abbreviation: Biomaterials
ISSN: 0142-9612
eISSN: 1878-5905
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: NCRR-UL1RR024979, NIDCD-P30 DC010362; DOI: 10.13039/100000005, name: U.S. Department of Defense; DOI: 10.13039/100000181, name: Air Force Office of Scientific Research; DOI: 10.13039/100002583, name: American Hearing Research Foundation; DOI: 10.13039/100014037, name: National Defense Science and Engineering Graduate, award: 32 CFR 168a; DOI: 10.13039/100000001, name: National Science Foundation, award: CBET-0933450
Language: English
Date published: 01/2013
Academic Unit: Molecular Physiology and Biophysics; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
Record Identifier: 9984003927802771

Metrics

25 Record Views

53 Times Cited - Web of Science