Journal article
Photoprotective effects of bucillamine against UV-induced damage in an SKH-1 hairless mouse model
Photochemistry and photobiology, Vol.84(2), pp.477-483
03/01/2008
DOI: 10.1111/j.1751-1097.2007.00288.x
PMID: 18266821
Abstract
UVB exposure of skin results in various biologic responses either through direct or indirect damage to DNA and non-DNA cellular targets via the formation of free radicals, reactive oxygen species (ROS) and inflammation. Bucillamine [N-(2-mercapto-2-methylpropionyl)-L-cysteine] is a cysteine-derived compound that can replenish endogenous glutathione due to its two donatable thiol groups, and functions as an antioxidant. In this study, we investigated the effects of bucillamine on UVB-induced photodamage using the SKH-1 hairless mouse model. We have demonstrated that UVB exposure (two consecutive doses, 230 mJ cm(-2)) on the dorsal skin of SKH-1 mice induced inflammatory responses (edema, erythema, dermal infiltration of leukocytes, dilated blood vessels) and p53 activation as early as 6 h after the last UVB exposure. Bucillamine pretreatment (20 mg kg(-1) of body weight, administered subcutaneously) markedly attenuated UVB-mediated inflammatory responses and p53 activation. We have also demonstrated that the stabilization and upregulation of p53 by UVB correlated with phosphorylation of Ser-15 and Ser-20 residues of p53 protein and that bucillamine pretreatment attenuated this effect. We propose that bucillamine has potential to be effective as a photoprotective agent for the management of pathologic conditions elicited by UV exposure.
Details
- Title: Subtitle
- Photoprotective effects of bucillamine against UV-induced damage in an SKH-1 hairless mouse model
- Creators
- Adil Anwar - University of Colorado Anschutz Medical CampusMallikarjuna Gu - University of Colorado HealthSara Brady - University of Colorado Anschutz Medical CampusLubna Qamar - University of Colorado Anschutz Medical CampusKian Behbakht - University of Colorado Anschutz Medical CampusYiqun G. Shellman - University of Colorado Anschutz Medical CampusRajesh Agarwal - University of Colorado HealthDavid A. Norris - University of Colorado Anschutz Medical CampusLawrence D. Horwitz - University of Colorado Anschutz Medical CampusMayumi Fujita - University of Colorado Anschutz Medical Campus
- Resource Type
- Journal article
- Publication Details
- Photochemistry and photobiology, Vol.84(2), pp.477-483
- Publisher
- Wiley
- DOI
- 10.1111/j.1751-1097.2007.00288.x
- PMID
- 18266821
- ISSN
- 0031-8655
- eISSN
- 1751-1097
- Number of pages
- 7
- Language
- English
- Date published
- 03/01/2008
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656605002771
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