Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin

Reshma Bhowmick; Mei Li; Jun Sun; Sheila A Baker; Christine Insinna; Joseph C Besharse

doi:10.1111/j.1600-0854.2009.00896.x

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Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin

Journal article

Open access

Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin

Reshma Bhowmick, Mei Li, Jun Sun, Sheila A Baker, Christine Insinna and Joseph C Besharse

Traffic (Copenhagen, Denmark), Vol.10(6), pp.648-663

06/2009

DOI: 10.1111/j.1600-0854.2009.00896.x

PMCID: PMC2827254

PMID: 19302411

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Abstract

Intraflagellar transport (IFT) provides a mechanism for the transport of cilium-specific proteins, but the mechanisms for linkage of cargo and IFT proteins have not been identified. Using the sensory outer segments (OS) of photoreceptors, which are derived from sensory cilia, we have identified IFT-cargo complexes containing IFT proteins, kinesin 2 family proteins, two photoreceptor-specific membrane proteins, guanylyl cyclase 1 (GC1, Gucy2e) and rhodopsin (RHO), and the chaperones, mammalian relative of DNAJ, DnajB6 (MRJ), and HSC70 (Hspa8). Analysis of these complexes leads to a model in which MRJ through its binding to IFT88 and GC1 plays a critical role in formation or stabilization of the IFT-cargo complexes. Consistent with the function of MRJ in the activation of HSC70 ATPase activity, Mg-ATP enhances the co-IP of GC1, RHO, and MRJ with IFT proteins. Furthermore, RNAi knockdown of MRJ in IMCD3 cells expressing GC1-green fluorescent protein (GFP) reduces cilium membrane targeting of GC1-GFP without apparent effect on cilium elongation.

Immunohistochemistry

Amino Acid Sequence

Two-Hybrid System Techniques

Animals

Molecular Chaperones - metabolism

Molecular Sequence Data

Guanylate Cyclase - metabolism

Mice

Photoreceptor Cells, Vertebrate - metabolism

Rhodopsin - metabolism

Details

Title: Subtitle: Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin
Creators: Reshma Bhowmick - Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Mei Li
Jun Sun
Sheila A Baker
Christine Insinna
Joseph C Besharse
Resource Type: Journal article
Publication Details: Traffic (Copenhagen, Denmark), Vol.10(6), pp.648-663
DOI: 10.1111/j.1600-0854.2009.00896.x
PMID: 19302411
PMCID: PMC2827254
NLM abbreviation: Traffic
ISSN: 1398-9219
eISSN: 1600-0854
Publisher: England
Grant note: R01 EY003222-29 / NEI NIH HHS P30-EY01931 / NEI NIH HHS EY03222 / NEI NIH HHS R01 EY003222 / NEI NIH HHS T32 EY014537 / NEI NIH HHS P30 EY001931 / NEI NIH HHS T32-EY014537 / NEI NIH HHS
Language: English
Date published: 06/2009
Academic Unit: Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
Record Identifier: 9984025287302771

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