Phr1 regulates retinogeniculate targeting independent of activity and ephrin-A signalling

Susan M Culican; A Joseph Bloom; Joshua A Weiner; Aaron DiAntonio

doi:10.1016/j.mcn.2009.04.001

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Phr1 regulates retinogeniculate targeting independent of activity and ephrin-A signalling

Journal article

Open access

Peer reviewed

Phr1 regulates retinogeniculate targeting independent of activity and ephrin-A signalling

Susan M Culican, A Joseph Bloom, Joshua A Weiner and Aaron DiAntonio

Molecular and cellular neurosciences, Vol.41(3), pp.304-312

07/2009

DOI: 10.1016/j.mcn.2009.04.001

PMCID: PMC2697264

PMID: 19371781

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Abstract

Proper functioning of the mammalian visual system requires that connections between the eyes and their central targets develop precisely. At birth, axons from the two eyes project to broad, overlapping regions of the dorsal-lateral geniculate nucleus (dLGN). In the adult, retinal axons segregate into distinct monocular regions at stereotyped locations within the dLGN. This process is driven by both molecular cues and activity-dependent synaptic competition. Here we demonstrate that Phr1, an evolutionarily conserved regulator of synapse formation and axon guidance, defines a novel molecular pathway required for proper localization of retinogeniculate projections. Following conditional excision of Phr1 in the retina, eye-specific domains within the dLGN are severely disturbed, despite normal spontaneous retinal wave activity and monocular segregation. Although layer placement is dramatically altered, Phr1 mutant retinal axons respond to ephrin-A in vitro. These findings indicate that Phr1 is a key presynaptic regulator of retinogeniculate layer placement independent of activity, segregation, or ephrin-A signaling.

Visual Pathways - abnormalities

Optic Chiasm - embryology

Signal Transduction

Membrane Proteins - genetics

Geniculate Bodies - metabolism

Visual Pathways - metabolism

Axons - metabolism

Optic Chiasm - metabolism

Retina - embryology

Mice, Knockout

Geniculate Bodies - abnormalities

Intracellular Signaling Peptides and Proteins - deficiency

Retina - physiology

Animals

Axons - pathology

Ephrins - metabolism

Membrane Proteins - metabolism

Mice

Retina - pathology

Details

Title: Subtitle: Phr1 regulates retinogeniculate targeting independent of activity and ephrin-A signalling
Creators: Susan M Culican - Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA
A Joseph Bloom
Joshua A Weiner
Aaron DiAntonio
Resource Type: Journal article
Publication Details: Molecular and cellular neurosciences, Vol.41(3), pp.304-312
DOI: 10.1016/j.mcn.2009.04.001
PMID: 19371781
PMCID: PMC2697264
NLM abbreviation: Mol Cell Neurosci
ISSN: 1044-7431
eISSN: 1095-9327
Publisher: Elsevier BV; United States
Grant note: R01 DA020812-02 / NIDA NIH HHS R01 DA020812 / NIDA NIH HHS DA020812 / NIDA NIH HHS
Language: English
Date published: 07/2009
Academic Unit: Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
Record Identifier: 9983997438002771

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