Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Kai Jiao; John J Nau; Marc Cool; William M Gray; Jan S Fassler; Robert E Malone

doi:10.1002/yea.800

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Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Journal article

Peer reviewed

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Kai Jiao, John J Nau, Marc Cool, William M Gray, Jan S Fassler and Robert E Malone

Yeast (Chichester, England), Vol.19(2), pp.99-114

01/30/2002

DOI: 10.1002/yea.800

PMID: 11788965

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Abstract

REC102 is a meiosis-specific early exchange gene absolutely required for meiotic recombination in Saccharomyces cerevisiae. Sequence analysis of REC102 indicates that there are multiple potential regulatory elements in its promoter region, and a possible regulatory element in the coding region. This suggests that the regulation of REC102 may be complex and may include elements not yet reported in other meiotic genes. To identify potential cis-regulatory elements, phylogenetic footprinting analysis was used. REC102 homologues were cloned from other two Saccharomyces spp. and sequence comparison among the three species defined evolutionarily conserved elements. Deletion analysis demonstrated that the early meiotic gene regulatory element URS1 was necessary but not sufficient for proper regulation of REC102. Upstream elements, including the binding sites for Gcr1p, Yap1p, Rap1p and several novel conserved sequences, are also required for the normal regulation of REC102 as well as a Rap1p binding site located in the coding region. The data in this paper support the use of phylogenetic comparisions as a method for determining important sequences in complex promoters.

Base Sequence

Binding Sites

Cloning, Molecular

DNA-Binding Proteins - metabolism

DNA-Binding Proteins - physiology

Fungal Proteins - genetics

Gene Expression Regulation, Fungal

Genes, Fungal

Meiosis - genetics

Molecular Sequence Data

Promoter Regions, Genetic

Protein Binding

Recombinases

Recombination, Genetic

Repressor Proteins - metabolism

Repressor Proteins - physiology

Saccharomyces cerevisiae - chemistry

Saccharomyces cerevisiae - classification

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae Proteins

Sequence Alignment

Telomere-Binding Proteins

Details

Title: Subtitle: Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102
Creators: Kai Jiao - Department of Biological Sciences, University of Iowa, Iowa City, IA 52246, USA
John J Nau
Marc Cool
William M Gray
Jan S Fassler
Robert E Malone
Resource Type: Journal article
Publication Details: Yeast (Chichester, England), Vol.19(2), pp.99-114
DOI: 10.1002/yea.800
PMID: 11788965
ISSN: 0749-503X
eISSN: 1097-0061
Grant note: R01-GM35486 / NIGMS NIH HHS R01-GM56719 / NIGMS NIH HHS R01 GM056719 / NIGMS NIH HHS R01 GM056719-05 / NIGMS NIH HHS
Language: English
Date published: 01/30/2002
Academic Unit: Biology
Record Identifier: 9984217425602771

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