Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614

Timothy E Mattes; Nicholas V Coleman; Jim C Spain; James M Gossett

doi:10.1007/s00203-004-0749-2

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Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614

Journal article

Peer reviewed

Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614

Timothy E Mattes, Nicholas V Coleman, Jim C Spain and James M Gossett

Archives of microbiology, Vol.183(2), pp.95-106

02/2005

DOI: 10.1007/s00203-004-0749-2

PMID: 15599705

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Abstract

Nocardioides sp. strain JS614 utilizes vinyl chloride and ethene as carbon and energy sources. JS614 could be influential in natural attenuation and biogeochemical ethene cycling, and useful for bioremediation, biocatalysis and metabolic engineering, but a fundamental understanding of the physiological and genetic basis of vinyl chloride and ethene assimilation in strain JS614 is required. Alkene monooxygenase (AkMO) activity was demonstrated in whole-cell assays and epoxyalkane:coenzyme M transferase (EaCoMT) activity was detected in JS614 cell-free extracts. Pulsed-field gel electrophoresis revealed a 290-kb plasmid (pNoc614) in JS614. Curing experiments and PCR indicated that pNoc614 encodes vinyl chloride/ethene-degradation genes. JS614 vinyl chloride/ethene catabolic genes and flanking DNA (34.8 kb) were retrieved from a fosmid clone. AkMO and EaCoMT genes were found in a putative operon that included CoA transferase, acyl-CoA synthetase, dehydrogenase, and reductase genes. Adjacent to this gene cluster was a divergently transcribed gene cluster that encoded possible coenzyme M biosynthesis enzymes. Reverse transcription-PCR demonstrated the vinyl chloride- and ethene-inducible nature of several genes. Genes encoding possible plasmid conjugation, integration, and partitioning functions were also discovered on the fosmid clone.

Plasmids

Polymerase Chain Reaction

Biodegradation, Environmental

Carbon-Sulfur Lyases - genetics

Nocardiaceae - genetics

Base Sequence

Open Reading Frames

Vinyl Chloride - metabolism

Molecular Sequence Data

Oxygenases - genetics

Ethylenes - metabolism

Nocardiaceae - metabolism

Details

Title: Subtitle: Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614
Creators: Timothy E Mattes - Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, IA 52242, USA. tim-mattes@uiowa.edu
Nicholas V Coleman
Jim C Spain
James M Gossett - Cornell University
Resource Type: Journal article
Publication Details: Archives of microbiology, Vol.183(2), pp.95-106
Publisher: Germany
DOI: 10.1007/s00203-004-0749-2
PMID: 15599705
ISSN: 0302-8933
eISSN: 1432-072X
Language: English
Date published: 02/2005
Academic Unit: Civil and Environmental Engineering
Record Identifier: 9983992068002771

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