Journal article
Piperlongumine inhibits proliferation and survival of Burkitt lymphoma in vitro
Leukemia research, Vol.37(2), pp.146-154
02/2013
DOI: 10.1016/j.leukres.2012.11.009
PMCID: PMC3551475
PMID: 23237561
Abstract
Piperlongumine (PL), a pepper plant alkaloid from Piper longum, kills solid tumor cells in a highly selective, potent fashion. To evaluate whether PL may have similar effects on malignant blood cells, we determined the efficacy with which PL inhibits the B-lymphocyte derived neoplasm, Burkitt lymphoma (BL). Low micromolar concentrations of PL (IC50=2.8μM×8.5μM) curbed growth and survival of two EBV+ BL cell lines (Daudi, Raji) and two EBV BL cell lines (Ramos, DG-75), but left normal peripheral blood B-lymphocytes unharmed. PL-dependent cytotoxicity was effected in part by reduced NF-κB and MYC activity, with the former being caused by inhibition of IκBα degradation, nuclear translocation of p65, and binding of NF-κB dimers to cognate DNA sequences in gene promoters. In 4 of 4 BL cell lines, the NF-κB/MYC-regulated cellular target genes, E2F1 and MYB, were down regulated, while the stress sensor gene, GADD45B, was up regulated. The EBV-encoded oncogene, LMP-1, was suppressed in Daudi and Raji cells. Considering that NF-κB, MYC and LMP-1 play a crucial role in the biology of many blood cancers including BL, our results provide a strong preclinical rationale for considering PL in new intervention approaches for patients with hematologic malignancies.
Details
- Title: Subtitle
- Piperlongumine inhibits proliferation and survival of Burkitt lymphoma in vitro
- Creators
- Seong-Su Han - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA, USADong-Ju Son - Emory University, Department of Biomedical Engineering, Atlanta, GA, USAHwakyung Yun - Hanseo University, Department of Biological Sciences, Choognam, South KoreaNatalie L Kamberos - Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, IA, USASiegfried Janz - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Leukemia research, Vol.37(2), pp.146-154
- DOI
- 10.1016/j.leukres.2012.11.009
- PMID
- 23237561
- PMCID
- PMC3551475
- NLM abbreviation
- Leuk Res
- ISSN
- 0145-2126
- eISSN
- 1873-5835
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 02/2013
- Academic Unit
- Stead Family Department of Pediatrics; Pathology
- Record Identifier
- 9984083863102771
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