Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation

Yueting Shang; Tadashi Yoshida; Brad A. Amendt; James F. Martin; Gary K. Owens

doi:10.1083/jcb.200711145

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Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation

Journal article

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Peer reviewed

Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation

Yueting Shang, Tadashi Yoshida, Brad A. Amendt, James F. Martin and Gary K. Owens

The Journal of cell biology, Vol.181(3), pp.461-473

05/05/2008

DOI: 10.1083/jcb.200711145

PMCID: PMC2364692

PMID: 18458156

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Abstract

Mechanisms that control vascular smooth muscle cell (SMC) differentiation are poorly understood. We identify Pitx2 as a previously unknown homeodomain transcription factor that is rapidly induced in an in vitro model of SMC differentiation from multipotent stem cells. Pitx2 induces expression of multiple SMC differentiation marker genes by binding to a TAATC(C/T) cis-element, by interacting with serum response factor, and by increasing histone acetylation levels within the promoters of SMC differentiation marker genes. Suppression of Pitx2 reduces expression of SMC differentiation marker genes in the early stages of SMC differentiation in vitro, whereas Prx1, another homeodomain protein, regulates SMC differentiation marker genes in fully differentiated SMCs. Pitx2, but not Prx1, knockout mouse embryos exhibit impaired induction of SMC differentiation markers in the dorsal aorta and branchial arch arteries. Our results demonstrate that Pitx2 functions to regulate the early stages of SMC differentiation.

Cell Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation
Creators: Yueting Shang - University of Virginia
Tadashi Yoshida - University of Virginia
Brad A. Amendt - Texas A&M Health Science Center
James F. Martin - Texas A&M Health Science Center
Gary K. Owens - University of Virginia
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.181(3), pp.461-473
DOI: 10.1083/jcb.200711145
PMID: 18458156
PMCID: PMC2364692
NLM abbreviation: J Cell Biol
ISSN: 0021-9525
eISSN: 1540-8140
Publisher: Rockefeller Univ Press
Number of pages: 13
Grant note: P01HL19242; R37HL57353; P01 HL019242; R37 HL057353; R01HL38854; R01 HL038854 / NHLBI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) R37HL057353 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
Language: English
Date published: 05/05/2008
Academic Unit: Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984284339802771

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