Journal article
Placenta-Specific Protein 1 Expression in Human Papillomavirus 16/18-Positive Cervical Cancers Is Associated With Tumor Histology
International journal of gynecological cancer, Vol.27(4), pp.784-790
05/2017
DOI: 10.1097/IGC.0000000000000957
PMCID: PMC5405019
PMID: 28375929
Abstract
Expression of the trophoblast-specific gene placenta-specific protein 1 (PLAC1) has been detected in a wide variety of cancers. However, to date, PLAC1 expression has not been shown in cervical cancer. We have carried out a preliminary study that shows for the first time that PLAC1 is expressed in cervical cancers. A total of 16 primary cervical tumors were obtained from patients shown to be human papillomavirus (HPV) 16/18 positive. Total cellular RNA, genomic DNA, and total protein were purified from each tumor. These materials were then used to determine PLAC1 expression, TP53 mutation status, and p53 expression. The PLAC1 expression was demonstrated in all 16 primary cervical tumors. The highest levels of expression were found in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Moreover, the proportion of total PLAC1 message coming from the P1 promoter, also termed the distal or cancer promoter, was significantly greater in the more aggressive squamous and adenosquamous histologic types compared with adenocarcinomas. Finally, in spite of all 16 tumors being HPV-16/18 positive, 3 of 8 squamous cell cancers and 2 of 5 adenocarcinomas expressed wild-type p53 protein. Consistent with the recently shown suppression of the PLAC1P1 promoter by wild-type p53, these p53 positive tumors displayed among the lowest P1-specific PLAC1 expression levels. The PLAC1 expression has been demonstrated for the first time in cervical cancers. This preliminary study has further revealed a complex relationship between PLAC1 expression, cervical cancer histologic type, p53, and HPV type that requires further investigation.
Details
- Title: Subtitle
- Placenta-Specific Protein 1 Expression in Human Papillomavirus 16/18-Positive Cervical Cancers Is Associated With Tumor Histology
- Creators
- Eric J Devor - University of IowaHenry D ReyesJesus Gonzalez-BosquetAkshaya WarrierSusan A KenzieNonye V IbikMarina D MillerBrandon M SchicklingMichael J GoodheartKristina W ThielKimberly K Leslie
- Resource Type
- Journal article
- Publication Details
- International journal of gynecological cancer, Vol.27(4), pp.784-790
- DOI
- 10.1097/IGC.0000000000000957
- PMID
- 28375929
- PMCID
- PMC5405019
- NLM abbreviation
- Int J Gynecol Cancer
- ISSN
- 1048-891X
- eISSN
- 1525-1438
- Publisher
- United States
- Grant note
- R01 CA099908 / NCI NIH HHS P30 CA086862 / NCI NIH HHS R01 CA184101 / NCI NIH HHS
- Language
- English
- Date published
- 05/2017
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983931819902771
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