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Plant cells contain calsequestrin
Journal article   Open access   Peer reviewed

Plant cells contain calsequestrin

Karl Heinz Krause, Mei Chou, Mitchell A Thomas, Richard D Sjolund and Kevin P Campbell
The Journal of biological chemistry, Vol.264(8), pp.4269-4272
1989
DOI: 10.1016/S0021-9258(18)83734-6
PMID: 2925646
url
https://doi.org/10.1016/S0021-9258(18)83734-6View
Published (Version of record) Open Access

Abstract

Calsequestrin is a high capacity low affinity Ca2+-binding protein thought to be essential for the function of the intracellular rapid releasable Ca2+ pool of a variety of animal cells. Here we show that two types of plant tissues, cultured Streptanthus tortuosus cells and spinach leaves, contain a form of calsequestrin. In subcellular fractions of S. tortuosus cells, Stains-all staining reveals a metachromatically blue-staining 56,000-Da protein enriched in the microsomal fraction. This protein shares several biochemical characteristics with animal calsequestrin: 1) it changes its apparent molecular weight with the pH; 2) it is able to bind 45Ca2+ on nitrocellulose transfers; and 3) it is recognized by antibodies against canine cardiac calsequestrin. Calsequestrin was also identified in spinach leaves using a direct extraction procedure that was developed for muscle calsequestrin. Thus, our results demonstrate that plant cells contain calsequestrin within a subcellular membrane fraction. These results also suggest that calsequestrin is an ubiquitous protein rather than being limited only to animal cells.
 Fundamental and applied biological sciences. Psychology Proteins Binding and carrier proteins Biological and medical sciences Analytical, structural and metabolic biochemistry

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