Journal article
Plasma Neurofilament Light and Glial Fibrillary Acidic Protein Levels over 30 days in a Porcine Model of Traumatic Brain Injury
Journal of neurotrauma, Vol.39(13-14), pp.935-943
07/2022
DOI: 10.1089/neu.2022.0070
PMCID: PMC9836679
PMID: 35369719
Abstract
In order to establish the clinical relevance of porcine model of TBI using the plasma biomarkers of injury with diffusion tensor imaging (DTI) over 30 days, we performed a randomized, blinded, preclinical trial using Yorkshire pigs weighing 7-10kg. Twelve pigs were subjected to Sham injury (n=5) by skin incision, or traumatic brain injury (n=7) by controlled cortical impact. Blood samples were collected before the injury, then at approximately 5-day intervals until 30 days. Both groups also had diffusion tensor imaging (DTI) at 24 hours and at 30 days after injury. Plasma samples were isolated and single molecule array (Simoa) was performed for glial fibrillary acidic protein (GFAP) and neurofilament light (NFL) levels. Afterwards, brain tissue samples were stained for β-APP. DTI showed FA decrease in the right corona radiata (ipsilateral to injury), contralateral corona radiata, and anterior corpus callosum at 1 day. At 30 days, ipsilateral corona radiata showed decreased FA. Pigs with TBI also had increase in GFAP and NFL at 1-5 days after injury. Significant difference between Sham and TBI animals continued up to 20 days. Linear regression showed significant negative correlation between ipsilateral corona radiata FA and both NFL and GFAP levels at 1 day. To further validate the degree of axonal injury found in DTI, β-APP immunohistochemistry was performed on a perilesional tissue as well as corona radiata bilaterally. Variable degree of staining was found in ipsilateral corona radiata. Porcine model of TBI replicates the acute increase in plasma biomarkers seen in clinical TBI. Furthermore, long term white matter injury is confirmed in the areas such as the splenium and corona radiata. However, future study stratifying severe and mild TBI, as well as comparison with other subtypes of TBI such as diffuse axonal injury may be warranted.
Details
- Title: Subtitle
- Plasma Neurofilament Light and Glial Fibrillary Acidic Protein Levels over 30 days in a Porcine Model of Traumatic Brain Injury
- Creators
- Samuel Shin - University of PennsylvaniaMarco M Hefti - University of IowaVanessa M Mazandi - Children's Hospital of PhiladelphiaDavid A Issadore - University of PennsylvaniaDavid Meaney - University of PennsylvaniaAndrea L. C. Schneider - University of PennsylvaniaRamon Diaz-Arrastia - University of PennsylvaniaTodd J Kilbaugh - Children's Hospital of Philadelphia
- Resource Type
- Journal article
- Publication Details
- Journal of neurotrauma, Vol.39(13-14), pp.935-943
- DOI
- 10.1089/neu.2022.0070
- PMID
- 35369719
- PMCID
- PMC9836679
- NLM abbreviation
- J Neurotrauma
- ISSN
- 0897-7151
- eISSN
- 1557-9042
- Language
- English
- Electronic publication date
- 04/04/2022
- Date published
- 07/2022
- Academic Unit
- Pathology; Iowa Neuroscience Institute
- Record Identifier
- 9984231870502771
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