Journal article
Plasma membrane Ca 2+-ATPase isoforms: distribution of mRNAs in rat brain by in situ hybridization
Brain research. Molecular brain research, Vol.16(3), pp.223-231
1992
DOI: 10.1016/0169-328X(92)90229-5
Abstract
Several mRNAs which encode for isoforms of the plasma membrane Ca
2+-transport ATPase (PMCA) are present in adult rat brain. Using in situ hybridization with antisense oligonucleotide probes we found complex patterns of specific hybridization for three isoforms (PMCA1–3). Each rat brain region studied exhibited a distinct pattern of expression of isoforms. PMCA1 mRNA, which is widely distributed in rat tissues, was highest in CA1 pyramidal cells of hippocampus and very low in hypothalamic nuclei, cerebellum and choroid plexus. PMCA2 mRNA was highest in Purkinje cells of cerebellum and low in caudate-putamen, hypothalamic nuclei, habenula and choroid plexus. The highest levels of PMCA3 mRNA were found in habenula and choroid plexus. The PMCA1–3 isoforms appeared to be expressed primarily in neurons since hybridization was detected neither in white matter nor in regions rich in astrocytes. In different regions, different levels of expression of each PMCA mRNA may underlie specialized requirements for calcium homeostasis in specific neurons.
Details
- Title: Subtitle
- Plasma membrane Ca 2+-ATPase isoforms: distribution of mRNAs in rat brain by in situ hybridization
- Creators
- William L. Stahl - Veterans Health AdministrationThomas J. Eakin - Veterans Affairs Medical Center, Seattle, WA 98108 USAJames W.M. Owens - Veterans Affairs Medical Center, Seattle, WA 98108 USAJohn F. Breininger - Veterans Affairs Medical Center, Seattle, WA 98108 USAPeter E. Filuk - Veterans Affairs Medical Center, Seattle, WA 98108 USAWilliam R. Anderson - Veterans Affairs Medical Center, Seattle, WA 98108 USA
- Resource Type
- Journal article
- Publication Details
- Brain research. Molecular brain research, Vol.16(3), pp.223-231
- Publisher
- Elsevier B.V; AMSTERDAM
- DOI
- 10.1016/0169-328X(92)90229-5
- ISSN
- 0169-328X
- eISSN
- 1872-6941
- Language
- English
- Date published
- 1992
- Academic Unit
- Stead Family Department of Pediatrics; Neurology (Pediatrics)
- Record Identifier
- 9984701654202771
Metrics
3 Record Views