Plasma proteomic analysis of stable coronary artery disease indicates impairment of reverse cholesterol pathway

Trayambak Basak; Vinay Singh Tanwar; Gourav Bhardwaj; Nitin Bhardwaj; Shadab Ahmad; Gaurav Garg; V Sreenivas; Ganesan Karthikeyan; Sandeep Seth; Shantanu Sengupta

doi:10.1038/srep28042

Back

Plasma proteomic analysis of stable coronary artery disease indicates impairment of reverse cholesterol pathway

Journal article

Open access

Peer reviewed

Plasma proteomic analysis of stable coronary artery disease indicates impairment of reverse cholesterol pathway

Trayambak Basak, Vinay Singh Tanwar, Gourav Bhardwaj, Nitin Bhardwaj, Shadab Ahmad, Gaurav Garg, V Sreenivas, Ganesan Karthikeyan, Sandeep Seth and Shantanu Sengupta

Scientific reports, Vol.6(1), pp.28042-28042

06/28/2016

DOI: 10.1038/srep28042

PMCID: PMC4923873

PMID: 27350024

Files and links (1)

url

https://doi.org/10.1038/srep28042View

Published (Version of record) Open Access

Abstract

Coronary artery disease (CAD) is one of the largest causes of death worldwide yet the traditional risk factors, although useful in identifying people at high risk, lack the desired predictive accuracy. Techniques like quantitative plasma proteomics holds immense potential to identify newer markers and this study (conducted in three phases) was aimed to identify differentially expressed proteins in stable CAD patients. In the first (discovery) phase, plasma from CAD cases (angiographically proven) and controls were subjected to iTRAQ based proteomic analysis. Proteins found to be differentially expressed were then validated in the second and third (verification and validation) phases in larger number of (n = 546) samples. After multivariate logistic regression adjusting for confounding factors (age, diet, etc.), four proteins involved in the reverse cholesterol pathway (Apo A1, ApoA4, Apo C1 and albumin) along with diabetes and hypertension were found to be significantly associated with CAD and could account for approximately 88% of the cases as revealed by ROC analysis. The maximum odds ratio was found to be 6.70 for albumin (p < 0.0001), followed by Apo AI (5.07, p < 0.0001), Apo CI (4.03, p = 0.001), and Apo AIV (2.63, p = 0.003). Down-regulation of apolipoproteins and albumin implicates the impairment of reverse cholesterol pathway in CAD.

Multidisciplinary Sciences

Science & Technology

Science & Technology - Other Topics

Details

Title: Subtitle: Plasma proteomic analysis of stable coronary artery disease indicates impairment of reverse cholesterol pathway
Creators: Trayambak Basak - Academy of Scientific and Innovative Research
Vinay Singh Tanwar - Institute of Genomics and Integrative Biology
Gourav Bhardwaj - Institute of Genomics and Integrative Biology
Nitin Bhardwaj - Institute of Genomics and Integrative Biology
Shadab Ahmad - Institute of Genomics and Integrative Biology
Gaurav Garg - Institute of Genomics and Integrative Biology
V Sreenivas - All India Institute of Medical Sciences
Ganesan Karthikeyan - All India Institute of Medical Sciences
Sandeep Seth - All India Institute of Medical Sciences
Shantanu Sengupta - Academy of Scientific and Innovative Research
Resource Type: Journal article
Publication Details: Scientific reports, Vol.6(1), pp.28042-28042
DOI: 10.1038/srep28042
PMID: 27350024
PMCID: PMC4923873
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: Springer Nature
Number of pages: 11
Grant note: BSC 0122 / Council of Scientific and Industrial Research (CSIR) under the XII FYP titled "CARDIOMED" BSC0122 / Council of Scientific and Industrial Research (CSIR), Ministry of Science and Technology, Govt of India, India CSIR; Council of Scientific & Industrial Research (CSIR) - India
Language: English
Date published: 06/28/2016
Academic Unit: Internal Medicine
Record Identifier: 9984359576102771

Metrics

14 Record Views

23 Times Cited - Web of Science