Journal article
Plasmid encoding miRNA-200c delivered by CaCO3-based nanoparticles enhances rat alveolar bone formation
Nanomedicine, Vol.17(19), pp.1339-1354
09/20/2022
DOI: 10.2217/nnm-2022-0151
PMCID: PMC9706369
PMID: 36125080
Abstract
Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c-induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO3 nanoparticles. Materials & methods: The CaCO3/miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c-induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO3 nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO3/miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO3 nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO3/miR-200c to craniofacial bone defects.
Details
- Title: Subtitle
- Plasmid encoding miRNA-200c delivered by CaCO3-based nanoparticles enhances rat alveolar bone formation
- Creators
- Matthew T Remy - University of IowaQiong Ding - University of IowaTadkamol Krongbaramee - University of IowaJue Hu - University of IowaAndrés V Mora MataAmanda J Haes - University of IowaBrad A Amendt - University of IowaHongli Sun - University of IowaMarisa R Buchakjian - Roy J. and Lucille A. Carver College of MedicineLiu Hong - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Nanomedicine, Vol.17(19), pp.1339-1354
- DOI
- 10.2217/nnm-2022-0151
- PMID
- 36125080
- PMCID
- PMC9706369
- NLM abbreviation
- Nanomedicine (Lond)
- ISSN
- 1743-5889
- eISSN
- 1748-6963
- Grant note
- DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE026433 (L Hong), R01DE029159 (H Sun), R4DE027569 (BA Amend)
- Language
- English
- Date published
- 09/20/2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Prosthodontics; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Chemistry; Dental Research; Otolaryngology
- Record Identifier
- 9984296182002771
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