Journal article
Plasmodium falciparum:Evaluation of Lactate Dehydrogenase in Monitoring Therapeutic Responses to Standard Antimalarial Drugs in Nigeria
Experimental parasitology, Vol.87(3), pp.283-289
11/1997
DOI: 10.1006/expr.1997.4251
PMID: 9371095
Abstract
The correlation ofP. falciparumlactate dehydrogenase (pLDH) activities and patent infections was evaluated for monitoring therapeutic responses and drug resistance in 70 patients with microscopically confirmedP. falciparummalaria in Nigeria. Each patient was treated with standard dosages of artemether (53 patients), chloroquine (7 patients), sulfadoxine-pyrimethamine (6 patients), or halofantrine (4 patients). Response of infection to treatment was monitored by microscopic examination of thick and thin blood smears, clinical symptoms, and levels of pLDH activities in blood products. pLDH activity was determined using an antibody capture technique and 3-acetyl pyridine adenine dinucleotide developed to enhance sensitivity of the enzyme detection. All patients treated with artemether were cured while 5 patients treated with chloroquine, 1 treated with sulfadoxine-pyrimethamine, and 2 treated with halofantrine suffered recrudescent infections after treatment. pLDH activity was detected in blood products obtained from patients with patent or recrudescent infections determined by microscopy and clinical symptoms. Levels of pLDH activities in whole blood and packed cells from the patients correlated with qualitative detection of parasites in blood smears and in patients with high gametocyte counts. Gametocyte counts in the patients after treatment ranged from 40 gametocytes/μl of blood to 4923 gametocytes/μl of blood. There is a consistent relationship between patent infection and pLDH activities that could easily be determined in whole blood and packed cells from the patients. Further development of the procedure will enhance its valuable application in clinical management of drug-resistant malaria in the endemic areas.
Details
- Title: Subtitle
- Plasmodium falciparum:Evaluation of Lactate Dehydrogenase in Monitoring Therapeutic Responses to Standard Antimalarial Drugs in Nigeria
- Creators
- A.M.J. Oduola - University of IbadanG.O. Omitowoju - University of IbadanA. Sowunmi - University of IbadanM.T. Makler - United States Department of Veterans AffairsC.O. Falade - University of IbadanD.E. Kyle - Walter Reed Army Institute of ResearchF.A. Fehintola - University of IbadanO.A.T. Ogundahunsi - University of IbadanR.C. Piper - Flow Inc. Portland, Oregon, U.S.A.B.G. Schuster - Walter Reed Army Institute of ResearchW.K. Milhous - Walter Reed Army Institute of Research
- Resource Type
- Journal article
- Publication Details
- Experimental parasitology, Vol.87(3), pp.283-289
- Publisher
- Elsevier Inc
- DOI
- 10.1006/expr.1997.4251
- PMID
- 9371095
- ISSN
- 0014-4894
- eISSN
- 1090-2449
- Language
- English
- Date published
- 11/1997
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297503802771
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