Journal article
Plasticity within the niche ensures the maintenance of a Sox2 + stem cell population in the mouse incisor
Development (Cambridge), Vol.145(1), pp.dev155929-dev155929
01/08/2018
DOI: 10.1242/dev.155929
PMCID: PMC5825875
PMID: 29180573
Abstract
In mice, the incisors grow throughout the animal's life, and this continuous renewal is driven by dental epithelial and mesenchymal stem cells.
is a principal marker of the epithelial stem cells that reside in the mouse incisor stem cell niche, called the labial cervical loop, but relatively little is known about the role of the
stem cell population. In this study, we show that conditional deletion of
in the embryonic incisor epithelium leads to growth defects and impairment of ameloblast lineage commitment. Deletion of
specifically in
cells during incisor renewal revealed cellular plasticity that leads to the relatively rapid restoration of a
-expressing cell population. Furthermore, we show that
-expressing cells are a subpopulation of dental
cells that also arise from
cells during tooth formation. Finally, we show that the embryonic and adult
populations are regulated by distinct signalling pathways, which is reflected in their distinct transcriptomic signatures. Together, our findings demonstrate that a
stem cell population can be regenerated from
cells, reinforcing its importance for incisor homeostasis.
Details
- Title: Subtitle
- Plasticity within the niche ensures the maintenance of a Sox2 + stem cell population in the mouse incisor
- Creators
- Maria Sanz-Navarro - Orthodontics, Department of Oral and Maxillofacial Diseases, University of Helsinki, 00290 Helsinki, FinlandKerstin Seidel - Department of Orofacial Sciences and Program in Craniofacial Biology, UCSF, San Francisco, CA 94143, USAZhao Sun - Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, The University of Iowa, Iowa City, IA 52242, USALudivine Bertonnier-Brouty - Département de Biologie, École Normale Supérieure de Lyon, Université de Lyon, 69007 Lyon, FranceBrad A Amendt - College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAOphir D Klein - Department of Pediatrics and Institute for Human Genetics, University of California San Francisco, San Francisco, CA 94143, USAFrederic Michon - Keele Medical School and Institute for Science and Technology in Medicine, Keele University, Keele ST5 5BG, UK
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.145(1), pp.dev155929-dev155929
- DOI
- 10.1242/dev.155929
- PMID
- 29180573
- PMCID
- PMC5825875
- NLM abbreviation
- Development
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Publisher
- England
- Grant note
- R35 DE026602 / NIDCR NIH HHS
- Language
- English
- Date published
- 01/08/2018
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984025456102771
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