Journal article
Platelet-derived CD154 enables T-cell priming and protection against Listeria monocytogenes challenge
Blood, Vol.111(7), pp.3684-3691
Immunobiology
04/01/2008
DOI: 10.1182/blood-2007-05-091728
PMCID: PMC2275027
PMID: 18256321
Abstract
Collagen exposure in tissue activates platelets, initiates wound healing, and modulates adaptive immunity. In this report, data are presented to demonstrate a requirement for platelet-derived CD154 for both collagen-induced augmentation of T-cell immunity and induction of pro-tective immunity to
Listeria
challenge. Specifically, we demonstrate that Ad5 encoding the membrane-bound form of ovalbumin (Ad5-mOVA) delivered in collagen induces higher ovalbumin-specific cytotoxic T lymphocyte (CTL) activity in a dose-dependent manner compared with Ad5-mOVA delivered in PBS. Increased CTL activity was dependent on the ability of platelets to respond to collagen and to express CD154. Furthermore, mice immunized with low-dose Ad5-mOVA in collagen were able to control a challenge of
Listeria monocytogenes
recombinant for ovalbumin expression (Lm-OVA), whereas mice immunized with low-dose Ad5-mOVA in PBS were not. These data indicate that in a physiologic setting that mimics wounding, platelets perform a sentinel function when antigen dose is too low to provoke an efficient immune response, and can enhance the generation of antigen-specific CD8 T cells that are functionally relevant to the host.
Details
- Title: Subtitle
- Platelet-derived CD154 enables T-cell priming and protection against Listeria monocytogenes challenge
- Creators
- Bennett D Elzey - Department of Comparative Pathobiology, Purdue University, West Lafayette, INNathan W Schmidt - Department of MicrobiologyScott A Crist - Department of Comparative Pathobiology, Purdue University, West Lafayette, INTimothy P Kresowik - Department of Urology, andJohn T Harty - Department of MicrobiologyBernhard Nieswandt - Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, andTimothy L Ratliff - Department of Comparative Pathobiology, Purdue University, West Lafayette, IN
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.111(7), pp.3684-3691
- Publisher
- American Society of Hematology; Washington, DC
- Series
- Immunobiology
- DOI
- 10.1182/blood-2007-05-091728
- PMID
- 18256321
- PMCID
- PMC2275027
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 04/01/2008
- Academic Unit
- Pathology; Urology; Internal Medicine
- Record Identifier
- 9984047851202771
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