Journal article
Pluronic/Heparin Nanoparticles for Chemo-Photodynamic Combination Cancer Therapy through Photoinduced Caspase‑3 Activation
ACS applied nano materials, Vol.1(6), pp.2943-2952
06/22/2018
DOI: 10.1021/acsanm.8b00572
Abstract
The Pluronic/heparin nanoparticles (NPs) were prepared for chemo-photodynamic combination cancer therapy through photoinduced caspase-3 activation. Doxorubicin (DOX) prodrug and methylene blue (MB) were assembled into a single structure by electrostatic interaction with heparin and further loaded into the NPs. MB is a photosensitizer, which can be used for photodynamic therapy, which can elicit cell death by producing reactive oxygen species (ROS). The DOX prodrug was DOX conjugated to a labile tetrapeptide (DEVD) by caspase-3 upon apoptosis. ROS was measured to verify ROS-mediated apoptosis. Membrane integrity test and flow cytometry analysis were performed to examine induced apoptosis. The tumor targeting and the in vivo antitumor efficacy were measured to evaluate chemo-photodynamic combination cancer therapy in tumor-bearing mice.
Details
- Title: Subtitle
- Pluronic/Heparin Nanoparticles for Chemo-Photodynamic Combination Cancer Therapy through Photoinduced Caspase‑3 Activation
- Creators
- Nisar Ul Khaliq - Korea UniversityDal Yong Park - Korea UniversityHye Jin Lee - Korea UniversityKeun Sang Oh - Korea UniversityJae Hong Seo - Korea University Guro HospitalSang Yoon Kim - University of Ulsan, College of MedicineChang Soon Hwang - TGel Bio Co., LtdTae-Hong Lim - The University of IowaSoon Hong Yuk - Korea University Guro Hospital
- Resource Type
- Journal article
- Publication Details
- ACS applied nano materials, Vol.1(6), pp.2943-2952
- Publisher
- American Chemical Society
- DOI
- 10.1021/acsanm.8b00572
- ISSN
- 2574-0970
- eISSN
- 2574-0970
- Grant note
- DOI: 10.13039/501100003625, name: Ministry of Health and Welfare, award: 1420390; DOI: 10.13039/501100003052, name: Ministry of Trade, Industry and Energy, award: 10077501; DOI: 10.13039/501100013129, name: Ministry of SMEs and Startups, award: S2561925
- Language
- English
- Date published
- 06/22/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984064246802771
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