Journal article
Polarity of DNA strand exchange promoted by recombination proteins of the RecA family
Proceedings of the National Academy of Sciences - PNAS, Vol.95(17), pp.9843-9848
08/18/1998
DOI: 10.1073/pnas.95.17.9843
PMCID: PMC21424
PMID: 9707563
Abstract
Homologs of
Escherichia coli
RecA recombination protein, which have been found throughout the living kingdom, promote homologous pairing and strand exchange. The nucleoprotein filament, within which strand exchange occurs, has been conserved through evolution, but conservation of the polarity of exchange and the significance of that directionality has not been settled. Using oligonucleotides as substrates, and assays based on fluorescence resonance energy transfer (FRET), we distinguished the biased formation of homologous joints at either end of duplex DNA from the subsequent directionality of strand exchange. As with
E. coli
RecA protein, the homologous Rad51 proteins from both
Homo sapiens
(HsRad51) and
Saccharomyces cerevisiae
(ScRad51) propagated DNA strand exchange preferentially in the 5′ to 3′ direction. The data suggest that 5′ to 3′ polarity is a conserved intrinsic property of recombination filaments.
Details
- Title: Subtitle
- Polarity of DNA strand exchange promoted by recombination proteins of the RecA family
- Creators
- Ravindra C Gupta - Departments ofEfim I Golub - Departments ofMarc S Wold - Departments ofCharles M Radding - Departments of
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.95(17), pp.9843-9848
- DOI
- 10.1073/pnas.95.17.9843
- PMID
- 9707563
- PMCID
- PMC21424
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 08/18/1998
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024403302771
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