Journal article
Polyanhydride Nanovaccines Induce Germinal Center B Cell Formation and Sustained Serum Antibody Responses
Journal of biomedical nanotechnology, Vol.12(6), pp.1303-1311
06/2016
DOI: 10.1166/jbn.2016.2242
PMCID: PMC5438750
PMID: 27319223
Abstract
Biodegradable polymeric nanoparticle-based subunit vaccines have shown promising characteristics by enhancing antigen presentation and inducing protective immune responses when compared with soluble protein. Specifically, polyanhydride nanoparticle-based vaccines (i.e., nanovaccines) have been shown to successfully encapsulate and release antigens, activate B and T cells, and induce both antibody- and cell-mediated immunity towards a variety of immunogens. One of the characteristics of strong thymus-dependent antibody responses is the formation of germinal centers (GC) and the generation of GC B cells, which is part of the T helper cell driven cellular response. In order to further understand the role of nanovaccines in the induction of antigen-specific immune responses, their ability to induce germinal center B cell formation and isotype switching and the effects thereof on serum antibody responses were investigated in these studies. Polyanhydride nanovaccines based on 1,6-bis(
p
-carboxyphenoxy)hexane and 1,8-bis(
p
-carboxyphenoxy)-3,6-dioxaoctane were used to subcutaneously administer a viral antigen. GC B cell formation and serum antibody responses induced by the nanovaccines were compared to that induced by alum-based vaccine formulations. It was demonstrated that a single dose of polyanhydride nanovaccines resulted in the formation of robust GCs and serum antibody in comparison to that induced by the alum-based formulation. This was attributed to the sustained release of antigen provided by the nanovaccines. When administered in a multiple dose regimen, the highest post-immunization titer and GC B cell number was enhanced, and the immune response induced by the nanovaccines was further sustained. These studies provide foundational information on the mechanism of action of polyanhydride nanovaccines.
Details
- Title: Subtitle
- Polyanhydride Nanovaccines Induce Germinal Center B Cell Formation and Sustained Serum Antibody Responses
- Creators
- Julia E Vela Ramirez - Iowa State UniversityLorraine T Tygrett - University of IowaJihua Hao - Case Western Reserve UniversityHabtom H Habte - Iowa State UniversityMichael W Cho - Iowa State UniversityNeil S Greenspan - Case Western Reserve UniversityThomas J Waldschmidt - University of IowaBalaji Narasimhan - Iowa State University
- Resource Type
- Journal article
- Publication Details
- Journal of biomedical nanotechnology, Vol.12(6), pp.1303-1311
- DOI
- 10.1166/jbn.2016.2242
- PMID
- 27319223
- PMCID
- PMC5438750
- NLM abbreviation
- J Biomed Nanotechnol
- ISSN
- 1550-7033
- eISSN
- 1550-7041
- Language
- English
- Date published
- 06/2016
- Academic Unit
- Pathology
- Record Identifier
- 9984186431702771
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