Journal article
Polyanhydride nanovaccine against H3N2 influenza A virus generates mucosal resident and systemic immunity promoting protection
npj vaccines, Vol.9(1), 96
05/31/2024
DOI: 10.1038/s41541-024-00883-3
PMCID: PMC11143372
PMID: 38822003
Abstract
Influenza A virus (IAV) causes significant morbidity and mortality worldwide due to seasonal epidemics and periodic pandemics. The antigenic drift/shift of IAV continually gives rise to new strains and subtypes, aiding IAV in circumventing previously established immunity. As a result, there has been substantial interest in developing a broadly protective IAV vaccine that induces, durable immunity against multiple IAVs. Previously, a polyanhydride nanoparticle-based vaccine or nanovaccine (IAV-nanovax) encapsulating H1N1 IAV antigens was reported, which induced pulmonary B and T cell immunity and resulted in cross-strain protection against IAV. A key feature of IAV-nanovax is its ability to easily incorporate diverse proteins/payloads, potentially increasing its ability to provide broad protection against IAV and/or other pathogens. Due to human susceptibility to both H1N1 and H3N2 IAV, several H3N2 nanovaccines were formulated herein with multiple IAV antigens to examine the "plug-and-play" nature of the polyanhydride nanovaccine platform and determine their ability to induce humoral and cellular immunity and broad-based protection similar to IAV-nanovax. The H3N2-based IAV nanovaccine formulations induced systemic and mucosal B cell responses which were associated with antigen-specific antibodies. Additionally, systemic and lung-tissue resident CD4 and CD8 T cell responses were enhanced post-vaccination. These immune responses corresponded with protection against both homologous and heterosubtypic IAV infection. Overall, these results demonstrate the plug-and-play nature of the polyanhydride nanovaccine platform and its ability to generate immunity and protection against IAV utilizing diverse antigenic payloads.
Details
- Title: Subtitle
- Polyanhydride nanovaccine against H3N2 influenza A virus generates mucosal resident and systemic immunity promoting protection
- Creators
- Christopher E Lopez - University of IowaZeb R Zacharias - University of IowaKathleen A Ross - Iowa State UniversityBalaji Narasimhan - Iowa State UniversityThomas J Waldschmidt - University of IowaKevin L Legge - Iowa State University
- Resource Type
- Journal article
- Publication Details
- npj vaccines, Vol.9(1), 96
- DOI
- 10.1038/s41541-024-00883-3
- PMID
- 38822003
- PMCID
- PMC11143372
- eISSN
- 2059-0105
- Grant note
- AI127565 / U.S. Department of Health & Human Services | National Institutes of Health (NIH) AI168001 / U.S. Department of Health & Human Services | National Institutes of Health (NIH) AI141196 / U.S. Department of Health & Human Services | National Institutes of Health (NIH) AI007533 / U.S. Department of Health & Human Services | National Institutes of Health (NIH) AI007485 / U.S. Department of Health & Human Services | National Institutes of Health (NIH)
- Language
- English
- Date published
- 05/31/2024
- Academic Unit
- Microbiology and Immunology; Pathology; Holden Comprehensive Cancer Center
- Record Identifier
- 9984635156402771
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